TY - JOUR AU - Watanabe, Shumpei AU - Masangkay, Joseph S. AU - Nagata, Noriyo AU - Morikawa, Shigeru AU - Mizutani, Tetsuya AU - Fukushi, Shuetsu AU - Alviola, Phillip AU - Omatsu, Tsutomu AU - Ueda, Naoya AU - Iha, Koichiro AU - Taniguchi, Satoshi AU - Fujii, Hikaru AU - Tsuda, Shumpei AU - Endoh, Maiko AU - Kato, Kentaro AU - Tohya, Yukinobu AU - Kyuwa, Shigeru AU - Yoshikawa, Yasuhiro AU - Akashi, Hiroomi T1 - Bat Coronaviruses and Experimental Infection of Bats, the Philippines T2 - Emerging Infectious Disease journal PY - 2010 VL - 16 IS - 8 SP - 1217 SN - 1080-6059 AB - Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription–PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9–1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection. KW - Bat coronavirus KW - severe acute respiratory syndrome KW - SARS KW - experimental infection KW - bats KW - coronavirus KW - viruses KW - the Philippines KW - zoonoses KW - research KW - Philippines DO - 10.3201/eid1608.100208 UR - https://wwwnc.cdc.gov/eid/article/16/8/10-0208_article ER - End of Reference