Volume 17, Number 2—February 2011
Dispatch
Unusual Transmission of Plasmodium falciparum, Bordeaux, France, 2009
Table
Genotyping results for 2 Plasmodium falciparum isolates, France, 2009*
Locus |
Secondary case | Index case | |||
---|---|---|---|---|---|
Type |
Allele |
Type |
Allele |
||
Microsatellites† | |||||
7A11 | NA | 119 bp | NA | 119 bp | |
C4M79 | NA | 203 bp | NA | 203 bp | |
Pf2802 | NA | 139 bp | NA | 139 bp | |
Pf2689 |
NA |
87 bp |
NA |
87 bp |
|
Antigen genes‡ | |||||
msp-1 | Ro33 | 149 bp | Ro33 | 149 bp | |
msp-2 |
FC27 |
413 bp |
FC27 |
413 bp |
|
Drug resistance genes§ | |||||
pfcrt codon 76 | Mutated | ACA | Mutated | ACA | |
pfdhfr | |||||
Codon 16 | Wild-type | GCA | Wild-type | GCA | |
Codon 51 | Mutated | ATT | Mutated | ATT | |
Codon 59 | Mutated | CGT | Mutated | CGT | |
Codon 108 | Mutated | AAC | Mutated | AAC | |
Codon 164 | Wild-type | ATA | Wild-type | ATA |
*NA, not applicable; msp, merozoite surface protein; pfcrt, P. falciparum chloroquine resistance transporter; pfdhfr, P. falciparum dihydrofolate reductase.
†Size of amplified DNA fragment expressed.
‡Allelic family and size of amplified DNA fragment expressed.
§Single-nucleotide polymorphism. pfcrt codon 76 point mutation was genotyped by nested PCR and restriction fragment-length polymorphism analysis. pfdhfr point mutations at positions 16, 51, 59, 108, and 164 were genotyped by nested PCR and primer expansion. Underlining indicates nucleotide positions that can be mutated.