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Volume 17, Number 5—May 2011

Detection and Phylogenetic Characterization of Human Hepatitis E Virus Strains, Czech Republic

Petra VasickovaComments to Author , Michal Slany, Pavel Chalupa, Michal Holub, Radek Svoboda, and Ivo Pavlik
Author affiliations: Author affiliations: Veterinary Research Institute, Brno, Czech Republic (P. Vasickova, M. Slany, I. Pavlik); Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic (P. Chalupa, M. Holub); Faculty of Medicine Masaryk University and the Faculty Hospital Brno, Brno (R. Svoboda)

Main Article

Table A1

Characterization of the 10 patients tested in the study and detection of HEV RNA by nested RT-PCR*

Patient no. Age, y/sex Town of origin Possible risk factors Bilirubin, μmol/L ALT, μkat/L AST, μkat/L GGT μkat/L ALP, μkat/L Systemic disease Hospital care, d Isolate name ORF1† ORF2/3
1‡ 62/M A NS 152.00 38.40 19.30 5.60 4.83 NS 13 CZhHEV107-09
2‡ 66/M B Ate homemade slaughter products; used same knife and chopping board for raw and cooked meat; ate undercooked meat in sushi bar 110.00 40.78 8.72 15.37 4.73 NS 11 CZhHEV108-09 + +
3‡ 62/M B Used same knife and chopping board for raw and cooked meat 614.00 25.22 28.78 4.98 5.77 DM, IHD 41 CZhHEV113-09 + +
4‡ 75/M B NS 203.00 20.24 28.57 2.68 3.35 NS 15 CZhHEV117-09 + +
5‡ 77/F B Ate pork 77.00 78.87 51.78 8.92 6.92 NS 10 CZhHEV114-09 +
6‡ 59/M B NS 297.00 88.44 45.57 4.52 2.50 CLL 10 ND + +
7‡ 59/M C Ate raw meat 14.50 8.99 5.52 1.96 2.24 NS 8 ND
65/F C NS 36.30 2.32 0.90 8.76 4.83 BC, CH 11 ND
27/F C Visited China 5.80 1.89 2.08 1.03 1.40 NS 7 ND
10§ 53/M C NS 113.10 19.48 20.43 20.52 10.49 SM 14 CZhHEV197-09

*HEV, hepatitis E virus; RT-PCR, reverse transcription–PCR; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, gama-glutamyltransferase; ALP, alkaline phosphatase; ORF1, primers targeting open reading frame 1 of HEV genome; ORF2/3, primers targeting overlapping part of HEV ORF2 and ORF3; NS, not shown; DM, diabetes mellitus; IHD, ischemic heart disease; CLL, chronic lymphatic leukemia; ND, not detected; BC, breast cancer; CH, chronic hepatopathy; SM, smoldering myeloma.
†Specific PCR product sequenced.
‡Detection of immunoglobulin (Ig) G antibodies against HEV by EIAgen HEV IgM kit (Adaltis, Rome, Italy), EIAgen HEV IgG Kit (Adaltis) and Recomblot HEV IgM/IgG (Microgen GmbH, Neured, Germany).
§Detection of IgM antibodies against HEV by HEV IgM ELISA 3.0 (MP Diagnostics, Illkirch Cedex, France) and HEV ELISA (MP Diagnostics).

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Page created: August 19, 2011
Page updated: August 19, 2011
Page reviewed: August 19, 2011
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