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Volume 17, Number 6—June 2011
Dispatch

Possible Novel Nebovirus Genotype in Cattle, France

Jérôme Kaplon, Eric Guenau, Philippe Asdrubal, Pierre Pothier, and Katia Ambert-BalayComments to Author 
Author affiliations: Author affiliations: Hôpital Universitaire de Dijon, Dijon, France (J. Kaplon, P. Pothier, K. Ambert-Balay); Laboratoire Départemental de Côte d’Or, Dijon (E. Guenau, P. Asdrubal)

Main Article

Figure 1

Nebovirus phylogenetic tree based on the deduced 167-aa–length sequences covering the 3′ end polymerase region. Possible novel strain is shown in boldface. Sequence alignments and clustering were performed by the unweighted-pair group method by using arithmetic average with Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Bootstrap values calculated with 1,000 replicate trees are given at each node when >70%. Collapsed branches are made up of the following nebovirus strains

Figure 1. Nebovirus phylogenetic tree based on the deduced 167-aa–length sequences covering the 3′ end polymerase region. Possible novel strain is shown in boldface. Sequence alignments and clustering were performed by the unweighted-pair group method by using arithmetic average with Bionumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Bootstrap values calculated with 1,000 replicate trees are given at each node when >70%. Collapsed branches are made up of the following nebovirus strains from France: *A042/06/FR, A051/05/FR, A058/05/FR, A130–2/06/FR, A143–2/06/FR, A311–2/08/FR, A381/08/FR, A445–2/08/FR, and A448/08/FR; †A281/07/FR, A355/07/FR, A356/07/FR, A364/08/FR, and A365/08/FR. Partial polymerase sequences of the French nebovirus strains were submitted to the GenBank database under accession nos. GU259537–GU259569 (A complete list is available from the authors.) GenBank accession nos. of calicivirus reference strains used in this tree are the following: AY082890 (CV23-OH/00/US), AY082891 (Nebraska/80/US), NC_007916 (Newbury1/76/UK), AF097917 (Newbury2/76/UK), DQ228162 (PenrithC39/00/UK), DQ228160 (Penrith142/00/UK), DQ228161 (Penrith143/00/UK), DQ228157 (Penrith150/00/UK), DQ228165 (Starcross93/00/UK), and DQ228164 (Starcross117/00/UK). Underlined strain is an outgroup. Scale bar indicates the percentage of similarities between strains.

Main Article

Page created: August 03, 2011
Page updated: August 03, 2011
Page reviewed: August 03, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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