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Volume 17, Number 8—August 2011

Reston Ebolavirus Antibodies in Bats, the Philippines

Satoshi Taniguchi, Shumpei Watanabe, Joseph S. Masangkay, Tsutomu Omatsu, Tetsuro Ikegami, Phillip Alviola, Naoya Ueda, Koichiro Iha, Hikaru Fujii, Yoshiyuki Ishii, Tetsuya Mizutani, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Kyuwa, Hiroomi Akashi, Yasuhiro Yoshikawa, and Shigeru MorikawaComments to Author 
Author affiliations: Author affiliations: University of Tokyo, Tokyo, Japan (S. Taniguchi, S. Watanabe, N. Ueda, K. Iha, H. Fujii, Y. Ishii, S. Kyuwa, H. Akashi, Y. Yoshikawa); National Institute of Infectious Diseases, Tokyo (S. Taniguchi, S. Watanabe, T. Omatsu, K. Iha, T. Mizutani, S. Fukushi, M. Saijo, I. Kurane, S. Morikawa); University of the Philippines, Laguna, the Philippines (J.S. Masangkay, P. Alviola); University of Texas of Medical Branch, Galveston, Texas, USA (T. Ikegami)

Main Article


REBOV-specific IgG in Rousettus amplexicaudatus bats and other bats, the Philippines*

Bat ID Collection site ELISA optical density
IFA titer
1539 FD 2.13 –0.21 1,280 <20
1632 FQ1 0.88 0.2 <20 <20
1642 FQ1 0.36 5.22 <20 20
1643 FQ1 1.26 0.92 <20 <20
1651 FQ1 1.61 1.02 <20 <20
1657 FQ1 –0.45 1.69 <20 <20
1660 FQ1 3.8 2.51 640 <20

*Cutoff optical density of ELISA was 0.82 (sum of optical densities at serum dilutions of 1:100, 1:400, 1:1,600, and 1:6,400). Values in boldface are positive results. REBOV, Reston Ebolavirus; Ig, immunoglobulin; IFA, indirect immunofluorescence assay; ID, identification; NP, nucleoprotein; GP, glycoprotein; FD, forest of Diliman at the University of the Philippines Diliman campus; FQ1, forest at the Agricultural College in Province of Quezon, the Philippines. The other 9 R. amplexicaudatus bats collected at FQ1 had negative results for all assays. The following bat species also had negative results: 5 Eonycteris spelaea, 35 Cynopterus brachyotis, 38 Ptenochirus jagoli, 6 Haplonycteris fischeri, 2 Macroglossus minimus, 2 Rhinolophus rufus, 1 Rhinolophus arcuatus, 9 Emballonura alecto, 2 Pipistrellus javanicus, 5 Scotophilus kuhlii, 8 Miniopterus australis, 8 M. schreibersi, 1 M. tristis tritis, 1 Hipposideros diadema, 1 Myotis macrotarsus, and 1 bat of unknown species.

Main Article

Page created: August 15, 2011
Page updated: August 15, 2011
Page reviewed: August 15, 2011
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