Figure 2. . . . Pulsed-field gel electrophoresis (PFGE) of AseI digest patterns of patient and household isolates, New York, New York, USA, 2001–2011. A) Patient and household isolates. Lane 1, λ ladder; lane 2, patient no. 5 Mycobacterium avium isolate AG-P-1; lane 3, patient no. 5 household filter M. avium isolate AG-F-2-0-2; lane 4, patient no. 5 household filter M. avium isolate AG-F-2-I-1 (environmental isolates in lanes 3 and 4 are indistinguishable; patient isolate in lane 2 considered clonal with 2 environmental isolates [6 bands difference]); with digestion with XbaI, the 3 were considered closely related.); lane 5, patient no. 6 M. avium complex “X” cluster (MAC-X) isolate GG-P-1; lane 6, patient no. 6 household swab M. chimaera isolate GG-Sw-9–1 (despite overall similarity, isolates in lanes 5 and 6 belong to different species and differ by 10 bands and are therefore unrelated). B) Additional patient and isolate from the person’s household. Lane 1, patient no. 2 M. avium isolate BB-P-1; lane 2, patient no. 2 household water M. avium isolate BB-W-4–5; lane 3, λ ladder.