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Volume 18, Number 11—November 2012

Antigenic Diversity of Enteroviruses Associated with Nonpolio Acute Flaccid Paralysis, India, 2007–2009

C. Durga RaoComments to Author , Prasanna Yergolkar1, and K. Subbanna Shankarappa12
Author affiliations: Author affiliations: Indian Institute of Science, Bangalore, India (C.D. Rao, K.S. Shankarappa); National Institute of Virology–World Health Organization–South East Asia Region Polio Laboratory Network, Victoria Hospital, Bangalore (P. Yergolkar); National Institute of Virology, Bangalore (P. Yergolkar); Victoria Hospital, Bangalore (P. Yergolkar)

Main Article


Analysis of fecal samples in 2,786 children with AFP and in 780 healthy children for virus growth in RD cells and for viral protein 1 gene RT-PCR, India, 2007–2009*

State/RD cell status Total no. AFP samples (second samples) No. samples used for RNA extraction
Total no. (%) RD-positive patients No. (%) RT-PCR-positive patients/RD-positive or -negative patients
2007 2008 2009
Karnataka 929 (253)


NA 51 75 87 213/676 (31.51) 141/213 (66.2)


NA 48 60 32 0 4/158 (2.5)†

Healthy children

NA 240 250 290 20/780 (2.56)† 18/20 (90.0)†
Uttar Pradesh: positive 1,833 (39) 129 165 232 526/1,794 (29.32) 331/526 (62.93)
Kerala 334 (18)


NA 22 45 17 84/316 (26.6) 60/84 (71.7)


NA 18
Total 3,096 490 595 676 823/2,786 (29.54) 532/823 (64.64)

*AFP, acute flacid paralysis; RT-PCR, reverse transcription PCR; NA, not applicable.
†Not included in RD cell–positive or RT-PCR–positive samples from children with AFP.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of Horticultural Sciences, Bagalkot, India.

Page created: October 15, 2012
Page updated: October 15, 2012
Page reviewed: October 15, 2012
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