Figure 2. . . . Phylogenetic analysis of the partial N terminal capsid gene (339 bp) of SaV strains identified in a pediatric population in Leon, Nicaragua, March 2005–September 2006. The tree was constructed on the basis of the Kimura 2-parameter and neighbor-joining methods with MEGA5 software (www.megasoftware.net). Bootstrap values are shown at the branch nodes (values <50% are not shown). The black squares represent SaV reference strains GI–GV. For Nicaraguan strains, the number of the strain is given, followed by age in months, month and year of sample collection, and clinical status. A, mild; C, severe; Asympt, asymptomatic. Scale bar indicates nucleotide substitutions per site.