Volume 18, Number 12—December 2012
Research
Diagnostic Assays for Crimean-Congo Hemorrhagic Fever
Table 2
Characteristics of selected assays compared in study of CCHF diagnostic tools
Characteristics | IgM ELISA | IgG ELISA | IgM IFA | IgG IFA | qRT-PCR | LCD array | |
---|---|---|---|---|---|---|---|
Assay (manufacturer, location or reference) | VectoCrimea-CHF ELISA (Vector-Best, Novosibirsk, Russia) | Crimean-Congo Fever Mosaic 2 IFA (Euroimmun, Luebeck, Germany) | RealStar CCHFV RT-PCR Kit 1.2 (Altona-Diagnostics, Hamburg, Germany) | CCHF2006 1.5 LCD Kit (26) | |||
Reference no. | D-5054 | D-5056 | FI 279a-1010-2M | FI 279a-1010-2G | 181203 | NA | |
Target | CCHFV-specific IgM | CCHFV-specific IgG | CCHFV-specific IgM | CCHFV-specific IgG | CCHFV S segment | CCHFV S segment | |
Shelf life, mo | 9 | 9 | 18 | 18 | 12 | Unknown | |
Storage temperature, °C | 2 to 8 | 2 to 8 | 2 to 8 | 2 to 8 | −15 to −25 | 2 to 8, −20 | |
Quoted accuracy, % | |||||||
Sensitivity |
100 | 100 | 97.2 | 89.5 | Unknown | 100 | |
Specificity |
100 | 100 | 97.5 | 100 | Unknown | 100 | |
Sample type | Serum, plasma | Serum, plasma | Serum, plasma | Serum, plasma | RNA extract from serum or blood | RNA extract from serum or blood | |
Sample volume, µL | 10 | 10 | 5 | 5 | 10 | 10 | |
Minimum kit format (no. reactions) | 12 strips × 8 tests (96) | 12 strips × 8 tests (96) | 10 slides × 5 tests (50) | 10 slides × 5 tests (50) | 8 tubes × 12 tests (96) | 4 slides × 8 tests (32) | |
Price, Euros† | |||||||
Per kit |
139.2 | 139.2 | 326 | 326 | 1,200 | Unknown | |
Per reaction |
1.45 | 1.45 | 6.51 | 6.51 | 12.50 | Unknown | |
Estimated run time, min | 175 | 175 | 70 | 70 | 58 | 175 |
*CCHFV, Crimean-Congo hemorrhagic fever virus; IFA, immunofluorescence assay; qRT-PCR, quantitative reverse transcription PCR; LCD, low-cost, low-density; NA, not applicable; S segment, small segment.
†Does not include shipping costs.
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