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Volume 18, Number 12—December 2012

Diagnostic Assays for Crimean-Congo Hemorrhagic Fever

Jessica VanhomwegenComments to Author , Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
Author affiliations: Author affiliations: Institut Pasteur, Paris, France (J. Vanhomwegen, P. Dubois); European Centre for Disease Control, Stockholm, Sweden (J. Vanhomwegen, H. Zeller); National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal (M.J. Alves); Faculty of Medicine, Ljubljana, Slovenia (T. Avšič Županc, M. Korva, A. Saksida); Institute of Public Health, Tirana, Albania (S. Bino); Pasteur Institute of Iran, Tehran, Iran (S. Chinikar); Swedish Institute for Infectious Disease Control, Solna, Sweden (H. Karlberg, A. Mirazimi, M. Mousavi); Refik Saydam National Public Health Agency, Ankara, Turkey (G. Korukluoğlu); Shahid Beheshti University of Medical Sciences, Tehran, Iran (M. Mardani, B. Sharifi-Mood); Aristotle University of Thessaloniki, Thessaloniki, Greece (A. Papa, P. Sidira, K. Tsergouli); and Bundeswehr Institute of Microbiology, Munich, Germany (R. Wölfel)

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Table 4

Overall performance of assays compared in study of CCHF diagnostic tools

Parameter IgM serology
IgG serology
Genome detection
No. samples tested 138 90 137 92 71 70
No. true positive 43 31 41 31 39 40
No. false negative 6 2 10 5 10 8
No. true negative 88 57 86 56 21 21
No. false positive 1 0 0 0 1 1
Sensitivity, % (95% CI) 87.8 (75.2–95.3) 93.9 (79.8–99.3) 80.4 (66.9–90.2) 86.1 (70.5–95.3) 79.6 (65.7–89.8) 83.3 (69.8–92.5)
Specificity, % (95% CI) 98.9 (93.9–100.0) 100.0 (93.7–100.0)† 100.0 (95.8–100.0) 100.0 (93.6–100.0) 95.5 (77.2–99.9) 95.5 (77.2–99.9)

*CCHF, Crimean-Congo hemorrhagic fever; IFA, immunofluorescent assay; qRT-PCR, quantitative reverse transcription PCR; LCD, low-cost, low-density.
†One-sided 95% CI.

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Page created: November 21, 2012
Page updated: November 21, 2012
Page reviewed: November 21, 2012
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