Diagnostic Assays for Crimean-Congo Hemorrhagic Fever
Jessica Vanhomwegen

, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, Helen Karlberg, Gülay Korukluoğlu, Miša Korva, Masoud Mardani, Ali Mirazimi, Mehrdad Mousavi, Anna Papa, Ana Saksida, Batool Sharifi-Mood, Persofoni Sidira, Katerina Tsergouli, Roman Wölfel, Hervé Zeller, and Philippe Dubois
Author affiliations: Author affiliations: Institut Pasteur, Paris, France (J. Vanhomwegen, P. Dubois); European Centre for Disease Control, Stockholm, Sweden (J. Vanhomwegen, H. Zeller); National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal (M.J. Alves); Faculty of Medicine, Ljubljana, Slovenia (T. Avšič Županc, M. Korva, A. Saksida); Institute of Public Health, Tirana, Albania (S. Bino); Pasteur Institute of Iran, Tehran, Iran (S. Chinikar); Swedish Institute for Infectious Disease Control, Solna, Sweden (H. Karlberg, A. Mirazimi, M. Mousavi); Refik Saydam National Public Health Agency, Ankara, Turkey (G. Korukluoğlu); Shahid Beheshti University of Medical Sciences, Tehran, Iran (M. Mardani, B. Sharifi-Mood); Aristotle University of Thessaloniki, Thessaloniki, Greece (A. Papa, P. Sidira, K. Tsergouli); and Bundeswehr Institute of Microbiology, Munich, Germany (R. Wölfel)
Main Article
Table 4
Overall performance of assays compared in study of CCHF diagnostic tools
Parameter |
IgM serology
|
|
IgG serology
|
|
Genome detection
|
ELISA |
IFA |
|
ELISA |
IFA |
|
qRT-PCR |
LCD array |
No. samples tested |
138 |
90 |
|
137 |
92 |
|
71 |
70 |
No. true positive |
43 |
31 |
|
41 |
31 |
|
39 |
40 |
No. false negative |
6 |
2 |
|
10 |
5 |
|
10 |
8 |
No. true negative |
88 |
57 |
|
86 |
56 |
|
21 |
21 |
No. false positive |
1 |
0 |
|
0 |
0 |
|
1 |
1 |
Sensitivity, % (95% CI) |
87.8 (75.2–95.3) |
93.9 (79.8–99.3) |
|
80.4 (66.9–90.2) |
86.1 (70.5–95.3) |
|
79.6 (65.7–89.8) |
83.3 (69.8–92.5) |
Specificity, % (95% CI) |
98.9 (93.9–100.0) |
100.0 (93.7–100.0)† |
|
100.0 (95.8–100.0) |
100.0 (93.6–100.0) |
|
95.5 (77.2–99.9) |
95.5 (77.2–99.9) |
Main Article
Page created: November 21, 2012
Page updated: November 21, 2012
Page reviewed: November 21, 2012
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