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Volume 18, Number 7—July 2012
Dispatch

Potential International Spread of Multidrug-Resistant Invasive Salmonella enterica Serovar Enteritidis

Irene RodríguezComments to Author , M. Rosario Rodicio, Beatriz Guerra, and Katie L. Hopkins
Author affiliations: Federal Institute for Risk Assessment, Berlin, Germany (I. Rodríguez, B. Guerra); Universidad de Oviedo, Oviedo, Spain (M.R. Rodicio); and Health Protection Agency, London, UK (K.L. Hopkins)

Main Article

Table 2

Characteristics of Salmonella enterica serovar Enteritidis isolates harboring pUO-SeVR1-like plasmids, 2005–2010, UK*

Isolate no. Phage type Resistance phenotype/ genotype Class 1 integron† pSEV genes‡ MLVA MLST VR plasmid, kb
CNM4839/03 PT 14b AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5,
srgB, srgC, pefA, pefB, pefC, pefD 2-12-9-4-4-3-NA-8-8 ST11 100
H051860415 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ST1479 95
H070360201 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB 2-15-9-4-4-3-NA-8-8 ST1479 88
H070420137 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB 2-15-9-4-4-3-NA-8-8 ND 88
H073180204 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 92
H091340084 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-9-9-4-4-3-NA-8-8 ST11 90
H091800482 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 92
H095100307 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95
H100240198 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95
H101700366 PT 42 AMP, CHL, STR, SUL, TET, TMP/blaTEM-1, catA2, strA-strB, sul1, sul2, tet(A), dfrA7 700 bp/dfrA7 spvA, spvB, spvC, spvR, rsk, rck, mig-5, ΔsrgA, srgB, srgC, pefA, pefB, pefC, pefD 2-13-9-4-4-3-NA-8-8 ND 95

*pSEV, serovar-specific V plasmid of S.enterica serovar Enteritidis; MLVA, multilocus variable number tandem repeat analysis; MLST, multilocus sequence typing; VR, virulence-resistance; PT, phage type; NA, no amplification from this locus; AMP, ampicillin; CHL, chloramphenicol; STR streptomycin; SUL, sulfonamides; TET, tetracycline; TMP, trimethoprim; ST, sequence type; ND, not done.
†Size of the variable region amplified with the 5′CS and 3′CS primers (2).
‡All plasmids were positive for IncFIIA, IncFIB and the par locus. Two new primer pairs were devised for detection of srgA: srgAB-Fw1/Rv1 (5′-CGCCTTCCGTGTATGTCC/GCGAGTCACTCACCGACAG-3′) and srgAB-Fw2/Rv2 (5′-GTTGCACAGGAGTGGGAGTC/GTCCGGGTTCCATGTCAG-3′). The forward primers anneal at different positions within srgA; the reverse primers anneal at different positions within srgB.

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References
  1. Graham  SM. Nontyphoidal salmonellosis in Africa. Curr Opin Infect Dis. 2010;23:409–14. DOIPubMedGoogle Scholar
  2. Rodríguez  I, Rodicio  MR, Herrera-León  S, Echeita  A, Mendoza  MC. Class 1 integrons in multidrug-resistant non-typhoidal Salmonella enterica isolated in Spain between 2002 and 2004. Int J Antimicrob Agents. 2008;32:158–64. DOIPubMedGoogle Scholar
  3. Chu  C, Chiu  CH. Evolution of the virulence plasmids of non-typhoid Salmonella and its association with antimicrobial resistance. Microbes Infect. 2006;8:1931–6. DOIPubMedGoogle Scholar
  4. Rodicio  MR, Herrero  A, Rodríguez  I, García  P, Montero  I, Beutlich  J, Acquisition of antimicrobial resistance determinants by virulence plasmids specific for nontyphoid serovars of Salmonella enterica. Rev Med Microbiol. 2011;22:55–65. DOIGoogle Scholar
  5. Rodríguez  I, Guerra  B, Mendoza  MC, Rodicio  MR. pUO-SeVR1 is an emergent virulence-resistance complex plasmid of Salmonella enterica serovar Enteritidis. J Antimicrob Chemother. 2011;66:218–20. DOIPubMedGoogle Scholar
  6. Fierer  J. Extraintestinal Salmonella infections: the significance of the spv genes. Clin Infect Dis. 2001;32:519–20. DOIPubMedGoogle Scholar
  7. Kado  CI, Liu  S-T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981;145:1365–73.PubMedGoogle Scholar
  8. Carattoli  A, Bertini  A, Villa  L, Falbo  V, Hopkins  KL, Threlfall  EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63:219–28. DOIPubMedGoogle Scholar
  9. Ward  LR, de Sa  JD, Rowe  B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect. 1987;99:291–4. DOIPubMedGoogle Scholar
  10. Malorny  B, Junker  E, Helmuth  R. Multi-locus variable-number tandem repeat analysis for outbreak studies of Salmonella enterica serotype Enteritidis. BMC Microbiol. 2008;8:84. DOIPubMedGoogle Scholar
  11. Vaagland  H, Blomberg  B, Krüger  C, Naman  N, Jureen  R, Langeland  N. Nosocomial outbreak of neonatal Salmonella enterica serotype Enteritidis meningitis in a rural hospital in northern Tanzania. BMC Infect Dis. 2004;4:35. DOIPubMedGoogle Scholar
  12. Kariuki  S, Revathi  G, Kariuki  N, Kiiru  J, Mwituria  J, Hart  CA. Characterisation of community acquired non-typhoidal Salmonella from bacteremia and diarrhoeal infections in children admitted to hospital in Nairobi, Kenya. BMC Microbiol. 2006;6:101. DOIPubMedGoogle Scholar
  13. Akinyemi  KO, Philipp  W, Beyer  W, Böhm  R. Application of phage typing and pulsed-field gel electrophoresis to analyse Salmonella enterica isolates from a suspected outbreak in Lagos, Nigeria. J Infect Dev Ctries. 2010;4:828–33. DOIPubMedGoogle Scholar
  14. Krauland  MG, Marsh  JW, Paterson  DL, Harrison  LH. Integron-mediated multidrug resistance in a global collection of nontyphoidal Salmonella enterica isolates. Emerg Infect Dis. 2009;15:388–96. DOIPubMedGoogle Scholar
  15. Kingsley  RA, Msefula  CL, Thomson  NR, Kariuki  S, Holt  KE, Gordon  MA, Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype. Genome Res. 2009;19:2279–87. DOIPubMedGoogle Scholar

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