Figure. . Vero cells inoculated with Amblyomma tick extracts for isolation of rickettsiae. A) Rickettsiae-like organisms stained in red by Gimenez staining (original magnification ×400). B) Inoculated monolayer photographed under phase-contrast microscopy (original magnification ×400). C) Uninfected control monolayer under phase-contrast microscopy (original magnification ×400). D) Transmission electron microscopy of infected cells. Bar indicates 2 µm. N, nucleolus. E) Transmission electron microscopy image of intravacuolar bacteria. Bar indicates 250 nm. Arrows indicate vacuoles containing bacteria.