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Volume 19, Number 2—February 2013

Laboratory-based Surveillance for Hepatitis E Virus Infection, United States, 2005–2012

Jan DrobeniucComments to Author , Tracy Greene-Montfort, Ngoc-Thao Le, Tonya R. Mixson-Hayden, Lilia Ganova-Raeva, Chen Dong, Ryan T. Novak, Umid M. Sharapov, Rania A. Tohme, Eyasu Teshale, Saleem Kamili, and Chong-Gee Teo
Author affiliations: Author affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA

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Release date: Release date: January 23, 2013; Expiration date: January 23, 2014

Learning Objectives

Upon completion of this activity, participants will be able to:

•     Describe the percentage of hepatitis E among US patients with hepatitis who were seronegative for acute hepatitis A and B, including those who had and those who had not traveled abroad

•     Compare characteristics of nontravelers vs travelers with hepatitis E

•     Describe HEV genotypes among nontravelers vs travelers with hepatitis E.

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Shannon O’Connor, ELS, Technical Writer/Editor, Emerging Infectious Diseases. Disclosure: Shannon O’Connor has disclosed no relevant financial relationships.

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Laurie Barclay, MD, freelance writer and reviewer, Medscape, LLC. Disclosure: Laurie Barclay, MD, has disclosed no relevant financial relationships.


Disclosures: Jan Drobeniuc; Tracy Greene-Montfort; Le Ngoc-Thao; Tonya R. Mixson-Hayden; Lilia Ganova-Raeva; Chen Dong; Ryan T. Novak; Umid M. Sharapov; Rania A. Tohme; Eyasu Teshale; Saleem Kamili; and Chong-Gee Teo have disclosed no relevant financial relationships.



To investigate characteristics of hepatitis E cases in the United States, we tested samples from persons seronegative for acute hepatitis A and B whose clinical specimens were referred to the Centers for Disease Control and Prevention during June 2005–March 2012 for hepatitis E virus (HEV) testing. We found that 26 (17%) of 154 persons tested had hepatitis E. Of these, 15 had not recently traveled abroad (nontravelers), and 11 had (travelers). Compared with travelers, nontravelers were older (median 61 vs. 32 years of age) and more likely to be anicteric (53% vs. 8%); the nontraveler group also had fewer persons of South Asian ethnicity (7% vs. 73%) and more solid-organ transplant recipients (47% vs. 0). HEV genotype 3 was characterized from 8 nontravelers and genotypes 1 or 4 from 4 travelers. Clinicians should consider HEV infection in the differential diagnosis of hepatitis, regardless of patient travel history.

Hepatitis E in Africa, southern and central Asia, and Central America causes occasional outbreaks of jaundice and, between outbreaks, occurrences of sporadic jaundice. Primarily spread by waterborne transmission, the disease tends to resolve spontaneously, although fulminant hepatic failure can ensue (1). In eastern Asia and Europe, sporadic hepatitis E, whether imported after return from international travel or acquired indigenously, has been observed; the indigenous form is thought to be foodborne (2). Although the disease is largely self-limiting, in Europe, chronic hepatitis E, which may lead to cirrhosis, is increasingly recognized among solid-organ transplant recipients (SOTRs) (3).

The causative agent of hepatitis E is hepatitis E virus (HEV), of which 4 genotypes are found in humans. Genotypes 1 and 2 circulate in regions where waterborne transmission is common; genotype 3 is prevalent in eastern Asia and the West and genotype 4 in eastern Asia. Genotypes 1 and 2 infect humans, but genotypes 3 and 4 infect humans and animals, predominantly pigs (4).

In the United States, HEV imported into the country after travel to regions to which waterborne HEV transmission is endemic is well recognized (57). Recently, 21% of participants of the US-based Third National Health and Nutrition Examination Survey were found seropositive for IgG against HEV (8). This unexpectedly high prevalence rate would not be ascribable to imported HEV infection alone. Indeed, cases of hepatitis E unassociated with travel abroad have been observed in the United States, implying infection by indigenous HEV strains (914). Moreover, the increasing number of reports from Europe of hepatitis E among SOTRs (3,15) suggests that SOTRS in the United States might be similarly susceptible to the disease.

We report a study of demographic, clinical, travel-related, and virologic characteristics of persons with hepatitis E derived from a diverse patient base. Critical to this investigation was the application of a validated serologic assay for detecting IgM against HEV (16), the marker of recent HEV infection, as well as a real-time reverse transcription PCR (RT-PCR) that had been validated to detect, to high sensitivity, HEV RNA (17), which is an indicator of active HEV shedding. Together, these 2 assays enabled us to identify patients with incident hepatitis E.


Samples and Patients

The Centers for Disease Control and Prevention (CDC) conducts HEV testing of serum and stool samples referred by health care providers, public health departments, and diagnostic laboratories in the United States (18). Referrers are requested to fill out a standardized questionnaire of patients’ demographics, clinical and laboratory test features, and risks for HEV infection, including recent international travel and destinations visited; the completed questionnaire is submitted along with the test specimens (18). Persons whose specimens were received during June 2005–March 2012 and reported as being negative for IgM against hepatitis A virus and hepatitis B core antigen, regardless of positivity for IgG against hepatitis C virus, were considered for inclusion into the study.


An earlier, pangenotypic evaluation by CDC of 6 serologic assays for IgM against HEV identified the assay manufactured by Diagnostic Systems (Saronno, Italy) as having the best performance characteristics (16). Its diagnostic sensitivity and specificity were 98% and 95.2%, respectively, and its analytic sensitivity was 9 Walter Reed Units/mL. For this study, the assay was used to detect IgM against HEV in test samples. IgG against HEV was tested by applying an assay from the same manufacturer. Serum and stool samples were tested for HEV RNA by a real-time RT-PCR, capable of detecting HEV genotypes 1–4 to a sensitivity of 4 HEV genome-equivalents/mL, to amplify a 69-bp fragment in open reading frame (ORF) 3 of the HEV genome (19). Application of that assay enabled our laboratory to attain perfect detection scores in a recent international evaluation of 20 laboratories conducting HEV RNA testing (17). Samples found to be positive for HEV RNA were subjected to another RT-PCR to generate amplicons from a 258-bp segment from ORF1, which were then processed for nucleotide sequencing and phylogenetic analyses (20).


Distributions of variables were assessed by using the Kruskal-Wallis test and the χ2 test with the Yates correction or the Fisher exact test, as appropriate. Univariate and bivariate data analyses were conducted by using Epi Info (

Case Definition

A case of hepatitis E was defined as illness in a person in whom IgM and IgG against HEV in serum or HEV RNA in serum or stool samples were detected. A person in whom IgM but not IgG against HEV was detected in serum was excluded unless HEV RNA was found or IgG againstHEV was detected in follow-up serum samples. A person in whom IgG but not IgM against HEV was detected in serum samples was included if HEV RNA was found in serum or stool samples.


Of 154 persons whose specimens fulfilled the inclusion criteria, 26 (17%) met the case definition for hepatitis E. Case-patients were between 14–67 years of age (median 43 years); 19 (73%) were male. Fifteen (58%) were white, 9 (36%) South Asian, and 2 (8%) Hispanic. None were seropositive for IgG against HEV. Eighteen (69%) case-patients were jaundiced, and 7 (27%) were SOTRs, the allografts received being kidney (3), liver (2), kidney and pancreas (1), and heart and lungs (1). Fifteen case-patients (58%) who reported not having traveled outside the United States in the previous 2 months were classified as nontravelers; the remaining 11, who had traveled abroad, were classified as travelers.

The Table summarizes the demographic, clinical, and virologic data for individual case-patients. Compared with travelers, nontravelers were older (median age 61 vs. 32 years of age; p<0.05) and more likely to be anicteric (not jaundiced; 8/15 [53%] vs. 1/11 [8%]; p = 0.02). The nontraveler group also included fewer South Asians (1/15 [7%] vs. 8/11 [73%]; p<0.001) and more SOTRs (7/15 [47%] vs. 0; p = 0.02). Differences in sex distribution were not significant.

Three case-patients (NT6, NT7, and T9; Table) were documented to have fulminant hepatic failure. Two of them required liver transplantation; 1 died and 1 survived.


Thumbnail of Genetic relatedness among hepatitis E virus (HEV) strains identified in hepatitis E cases, United States. Phylogenetic tree was constructed from a segment of HEV open reading frame 1 generated in MEGA5 ( by using the neighbor-joining method. Country, year reported, and numeric or GenBank accession number assignment are denoted. Scale bar indicates genetic distance.

Figure. . . . Genetic relatedness among hepatitis E virus (HEV) strains identified in hepatitis E cases, United States. Phylogenetic tree was constructed from a segment of HEV open reading frame...

HEV RNA was amplified from 12 case-patients (46%). The rate of HEV RNA detection among SOTRs (5/7; 71%) was higher than that among non-SOTRs (7/19; 37%), but this difference was not significant. HEV genotype 1 was characterized from 3 travelers, genotype 3 from 8 nontravelers (including the 5 SOTRs), and genotype 4 from 1 traveler. The Figure displays the genetic diversity of HEV carried.


We identified 26 case-patients with hepatitis E in the United States. No distinction was made between acute and chronic hepatitis E. Whereas acute hepatitis among non-SOTRs was readily identifiable (most were jaundiced when test specimens were drawn), it was difficult to asses whether disease in SOTRs was at the acute or chronic stage during specimen sampling, because positivity for IgM against HEV or HEV RNA could reflect either stage (3). Thus, the case definition was kept broad to identify both stages of disease. As the study was not primarily prospective, the natural history of hepatitis E among the case-patients was largely unknown. Nevertheless, adverse outcomes could be documented for 3 case-patients, in whom fulminant hepatic failure developed.

Hepatitis E cases were found among persons who had not recently traveled abroad and those who had. Nontravelers tended to be older than travelers, a trend consistent with the finding recently reported by the Drug-Induced Liver Injury Network of 9 patients seropositive for IgM against HEV whose mean age was 67 years (21) and with similar observations in Japan and Europe (1,2,22). The higher proportion of anicteric persons in the nontraveler group reflects its inclusion of all SOTRs, which in Europe have been observed to have largely asymptomatic infections (3).

Nontravelers were infected exclusively by HEV genotype 3 strains. These strains clustered with HEV previously found in case-patients with nonimported acute hepatitis E (912,14) in the United States (Figure), suggesting that the nontravelers were infected by autochthonously circulating HEV. The similarity between HEV genotype 3 strains identified in nontravelers with those in swine (4) (Figure) suggests, but does not prove, HEV transmission linkage between humans and pigs (2). Evidence of HEV infection acquired after consumption of inadequately cooked meat and offal originating from pigs, boars, and deer has been reported from Japan (2) and France (23). Elsewhere, including the United States, evidence implicating non–travel-associated hepatitis E as a zoonosis remains weak (24).

The patient base from which hepatitis E cases were identified was nonselective, broad, and derived from multiple health care provider contexts. Nonetheless, data from this study were not generated from an established, systematic program of epidemiologic surveillance. Accordingly, the cases identified here may not fully represent the extent of hepatitis E in the United States. The larger number of cases among nontravelers likely reflects more persons living in the United States who do not travel abroad compared with those who do, and the many SOTRs identified with hepatitis E could be an overrepresentation resulting from increasing awareness among physicians of the predilection of the SOTR patient subpopulation to HEV infection (3,15). Future surveillance of hepatitis E may need to sample source populations from more diverse settings, such as gastroenterology/hepatology clinics (21), travel clinics (6), and the military (7). We recently reported findings from a study of HEV infection among immunocompromised patients other than SOTRs (25).

This study has provided insight into nonimported and imported hepatitis E in the United States. The nonimported form was observed to affect SOTRs, be able to lead to adverse outcomes, and be associated with infection by HEV genotype 3. The extent of nonimported hepatitis E in the United States merits further investigation, as does the role of autochthonous transmission of genotype 3 HEV strains. In clinical practice, entry of hepatitis E into the differential diagnosis of suspected hepatitis, regardless of the patient’s travel history, would be appropriate.

Dr Drobeniuc is a microbiologist in the Division of Viral Hepatitis, Centers for Disease Control and Prevention. His research interests include laboratory diagnostics, assay development, quality assurance, and epidemiology relating to viral hepatitis.



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Article Title:
Laboratory-based Surveillance for Hepatitis E Virus Infection, United States, 2005–2012

CME Questions

1. You are a consultant advising an HMO regarding the percentage of hepatitis E among US patients with hepatitis. Based on the study by Dr. Drobeniuc and colleagues, which of the following statements would most likely appear in your report?

A.        Hepatitis E was present in more than half of patients who were seronegative for acute hepatitis A and B

B.        Among patients with hepatitis E, only one quarter had recently traveled abroad

C.        Among patients with hepatitis E, half the patients had acute and half the patients had chronic hepatitis

D.        Hepatitis E virus (HEV) infection was determined by testing for IgM and IgG anti-HEV and for HEV RNA

2. Based on the study by Dr. Drobeniuc and colleagues, which of the following statements about group characteristics of nontravelers vs travelers with hepatitis E is most likely correct?

A.        Nontravelers were older than travelers

B.        Nontravelers were more likely than travelers to be jaundiced

C.        Nontravelers comprised fewer South Asians than travelers

D.        Nontravelers were less likely than travelers to be solid organ transplant recipients

3. Based on the study by Dr. Drobeniuc and colleagues, which of the following statements about HEV genotypes among nontravelers vs travelers with hepatitis E is most likely correct?

A.        Nontravelers were infected exclusively by HEV genotype 1 strains

B.        Nontravelers were infected by HEV genotype 3 and 4 strains

C.        Travelers were infected exclusively by HEV genotype 3 strains

D.        The findings suggest that the nontravelers were infected by HEV that was circulating autochthonously in the United States.

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Cite This Article

DOI: 10.3201/eid1902.120961

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Page created: January 22, 2013
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