Volume 19, Number 8—August 2013
Research
Aichi Virus in Sewage and Surface Water, the Netherlands
Table 1
Oligonucleotide sequences of the VP1 and VP3 primers developed and used for study of Aichi virus in sewage and surface water, the Netherlands*
Primer | Sequence, 5′→3′† | Nucleotide location‡ | PCR |
---|---|---|---|
AiV-VP3-F1 | CACACCGCCCCTGCGTCRGCCCTCGT | 2912–2937 | First-round |
AiV-VP1-F2 | CTCGATGCRCCMCAAGACACCGG | 3023–3045 | Nested |
AiV-VP1-F3 | GTGCTTCACRTACATCGCYGCGG | 3289–3311 | Nested |
AiV-VP1-R2 | CCTGACCAGTCCTCCCAWCCGAAGTA | 3552–3527 | Nested |
AiV-VP1-R1 | GAGAGCTGGAAGTCRAAGGG | 3651–3632 | First-round |
*VP, viral protein; AiV, Aichi virus; F, forward; R, reverse.
†R indicates A or G; M indicates A or C; Y indicates C or T; W indicates A or T.
‡Compared with Aichi virus reference strain (GenBank accession no. AB010145).