TY - JOUR AU - Tate, Jacqueline AU - Mijatovic-Rustempasic, Slavica AU - Tam, Ka Ian AU - Lyde, Freda AU - Payne, Daniel AU - Szilagyi, Peter AU - Edwards, Kathryn AU - Staat, Mary Allen AU - Weinberg, Geoffrey AU - Hall, Caroline AU - Chappell, James AU - McNeal, Monica AU - Gentsch, Jon AU - Bowen, Michael AU - Parashar, Umesh T1 - Comparison of 2 Assays for Diagnosing Rotavirus and Evaluating Vaccine Effectiveness in Children with Gastroenteritis T2 - Emerging Infectious Disease journal PY - 2013 VL - 19 IS - 8 SP - 1245 SN - 1080-6059 AB - We compared rotavirus detection rates in children with acute gastroenteritis (AGE) and in healthy controls using enzyme immunoassays (EIAs) and semiquantitative real-time reverse transcription PCR (qRT-PCR). We calculated rotavirus vaccine effectiveness using different laboratory-based case definitions to determine which best identified the proportion of disease that was vaccine preventable. Of 648 AGE patients, 158 (24%) were EIA positive, and 157 were also qRT-PCR positive. An additional 65 (10%) were qRT-PCR positive but EIA negative. Of 500 healthy controls, 1 was EIA positive and 24 (5%) were qRT-PCR positive. Rotavirus vaccine was highly effective (84% [95% CI 71%–91%]) in EIA-positive children but offered no significant protection (14% [95% CI −105% to 64%]) in EIA-negative children for whom virus was detected by qRT-PCR alone. Children with rotavirus detected by qRT-PCR but not by EIA were not protected by vaccination, suggesting that rotavirus detected by qRT-PCR alone might not be causally associated with AGE in all patients. KW - rotavirus KW - enzyme immunoassay KW - RT-PCR KW - viruses KW - children KW - gastroenteritis KW - diagnosis KW - acute gastroenteritis KW - United States DO - 10.3201/eid1908.130461 UR - https://wwwnc.cdc.gov/eid/article/19/8/13-0461_article ER - End of Reference