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Volume 20, Number 1—January 2014
Dispatch

Rapidly Fatal Hemorrhagic Pneumonia and Group A Streptococcus Serotype M1

Maria Santagati1, Teresa Spanu1, Marina Scillato, Rosaria Santangelo, Fabio Cavallaro, Vincenzo Arena, Giacomo Castiglione, Marco Falcone, Mario Venditti, and Stefania StefaniComments to Author 
Author affiliations: University of Catania, Catania, Italy (M. Santagati, M. Scillato, S. Stefani); Catholic University of the Sacred Heart, Rome, Italy (T. Spanu, R. Santangelo, F. Cavallaro, V. Arena); Vittorio Emanuele Hospital, Catania (G. Castiglione); University of Rome La Sapienza, Rome (M. Falcone, M. Venditti)

Main Article

Table

Primers used for identification of virulence genes by PCR for group C Streptococcus isolates*

Gene Primer name† Primer sequence, 5′→3′ Amplicon size, bp
Slo MS442 GGTAACCTTGTTACTGCTAATGCTGA 400
MS443 TAATGGAAATATCGACTGGTGTAGTG‡ 400
speA speA-fw CTCAACAAGACCCCGATCCAAG 500
speA-rew ATTTAGAAGGTCCATTAGTATATAGTTGC‡ 500
speB MS384 GGCATGTCCGCCTACTTTACCGA 800
MS385 CAGGTGCACGAAGCGCAGAAG‡ 800
speC MS410 TACTGATTTCTACTATTTCACCTATCATC 447
MS411 TCTGATTTTAAAGTCAATTTCCTGG‡ 447
speG MS412 GCTATGGAAGTCAATTAGCTTATGCAG 448
MS413 CCGATGTATAACGCGATTCCGA‡ 448
speI speI-up GGTCCGCCATTTTCAGGTAGTTT 516
speI-rew ACGCATACGAAATCATACCAGTAG‡ 516
speJ MS414 CACTCCTTGTACTAGATGAGGTTGC 508
MS415 ACGCATACGAAATCATACCAGTAG‡ 508
sagBC sagBC-fw GCAGCTAGTTGCTCAACATTTAATG 600
sagBC sagBC-rew CATAGGCAGTCGCCTGATTCC‡ 600
prtF MS400 CGGAGTATCAGTAGGACATGCGGA 882
prtF MS401 CTCCCACCAACATTGCTTAATCCA‡ 882
PAM PAM-fw GCAGACGACGCTAGAAATGAAGTA 900
PAM-rew CCTGCTTGTGGTGCTTGACCTTTAC‡ 900
Sof MS402 ATGCCTGGTTGGGTATCTTCGGT 406
MS403 AGAGAACAAAACGTTCTGCGCCTA‡ 406
Ssa ssa-fw GTAGTCAGCCTGACCCTACTCCAGAAC 621
ssa-rew ACTGATCAAATATTGCTGCAGGTGC‡ 621
sda1 MS431 GGGTCTATAAGAAAAGTGGGCAAAG 439
MS432 TGATCGTAAAGGTGGGATGCAGTA‡ 439

*slo, streptolysin O; sagA/BC, streptolysin S-associated gene A protein; smeZ-2 mitogenic exotoxin Z; speA, streptococcal pyrogenic toxin A; speB, streptococcal cysteine protease; speC, streptococcal pyrogenic toxin C; speG, streptococcal pyrogenic toxin G; speI, streptococcal pyrogenic toxin I; speJ; streptococcal pyrogenic toxin J; prtF, fibronectin-binding protein; PAM, plasminogen-binding protein; sof serum opacity factor; ssa streptococcal superantigen; sda1, streptodornase D.
†Primers indicated in boldface were created with the VectorNT program (Invitrogen, Carlsbad, CA, USA). Other primers were described by Santagati et al. (11).
‡Reverse primers.

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References
  1. Carapetis  JR, Steer  AC, Mulholland  EK, Weber  M. The global burden of group A streptococcal diseases. Lancet Infect Dis. 2005;5:685–94. DOIPubMedGoogle Scholar
  2. Steer  AC, Lamagni  T, Curtis  N, Carapetis  JR. Invasive group a streptococcal disease: epidemiology, pathogenesis and management. Drugs. 2012;72:1213–27. DOIPubMedGoogle Scholar
  3. Cole  JN, Barnett  TC, Nizet  V, Walker  MJ. Molecular insight into invasive group A streptococcal disease. Nat Rev Microbiol. 2011;9:724–36. DOIPubMedGoogle Scholar
  4. Henningham  A, Barnett  TC, Maamary  PG, Walker  MJ. Pathogenesis of group A streptococcal infections. Discov Med. 2012;13:329–42 .PubMedGoogle Scholar
  5. Friães  A, Pinto  FR, Silva-Costa  C, Ramirez  M, Melo-Cristino  J. Group A streptococci clones associated with invasive infections and pharyngitis in Portugal present differences in emm types, superantigen gene content and antimicrobial resistance. BMC Microbiol. 2012;12:280. DOIPubMedGoogle Scholar
  6. Lamagni  TL, Efstratiou  A, Vuopio-Varkila  J, Jasir  A, Schalén  C. The epidemiology of severe Streptococcus pyogenes associated disease in Europe. Euro Surveill. 2005;10:179–84 .PubMedGoogle Scholar
  7. Turner  CE, Dryden  M, Holden  MT, Davies  FJ, Lawrenson  RA, Farzaneh  L, Molecular analysis of an outbreak of lethal postpartum sepsis caused by Streptococcus pyogenes. J Clin Microbiol. 2013;51:2089–95. DOIPubMedGoogle Scholar
  8. Sumby  P, Porcella  SF, Madrigal  AG, Barbian  KD, Virtaneva  K, Ricklefs  SM, Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events. J Infect Dis. 2005;192:771–82. DOIPubMedGoogle Scholar
  9. Herwald  H, Cramer  H, Mörgelin  M, Russell  W, Sollenberg  U, Norrby-Teglund  A, M protein, a classical bacterial virulence determinant, forms complexes with fibrinogen that induce vascular leakage. Cell. 2004;116:367–79. DOIPubMedGoogle Scholar
  10. Uchiyama  S, Andreoni  F, Schuepbach  RA, Nizet  V, Zinkernagel  AS. DNase Sda1 allows invasive M1T1 group A Streptococcus to prevent TLR9-dependent recognition. PLoS Pathog. 2012;8:e1002736. DOIPubMedGoogle Scholar
  11. Santagati  M, Scillato  M, Patane  F, Aiello  C, Stefani  S. Bacteriocin-producing oral streptococci and inhibition of respiratory pathogens. FEMS Immunol Med Microbiol. 2012;65:23–31. DOIPubMedGoogle Scholar
  12. Lassalle  S, Hofman  V, Butori  C, Sicard  D, Hofman  P. Histological diagnosis of lung bacterial necrotizing infections: a study of four fulminant cases. Ann Pathol. 2005;25:349–56. DOIPubMedGoogle Scholar
  13. Walker  MJ, Hollands  A, Sanderson-Smith  ML, Cole  JN, Kirk  JK, Henningham  A, DNase Sda1 provides selection pressure for a switch to invasive group A streptococcal infection. Nat Med. 2007;13:981–5. DOIPubMedGoogle Scholar
  14. Kansal  RG, McGeer  A, Low  DE, Norrby-Teglund  A, Kotb  M. Inverse relation between disease severity and expression of the streptococcal cysteine protease, SpeB, among clonal M1T1 isolates recovered from invasive group A streptococcal infection cases. Infect Immun. 2000;68:6362–9 . DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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