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Volume 20, Number 12—December 2014
Research

Geographic Divergence of Bovine and Human Shiga Toxin–Producing Escherichia coli O157:H7 Genotypes, New Zealand1

Patricia JarosComments to Author , Adrian L. Cookson, Donald M. Campbell, Gail E. Duncan, Deborah Prattley, Philip E. Carter, Thomas E. Besser, Smriti Shringi, Steve Hathaway, Jonathan C. Marshall, and Nigel P. French
Author affiliations: Massey University, Palmerston North, New Zealand (P. Jaros, D. Prattley, J.C. Marshall, N.P. French); AgResearch Ltd, Palmerston North (A.L. Cookson); Ministry for Primary Industries, Wellington, New Zealand (D.M. Campbell, G.E. Duncan, S. Hathaway); Institute of Environmental Science and Research Ltd, Porirua, New Zealand (P. Carter); Washington State University, Pullman, Washington, USA (T.E. Besser, S. Shringi)

Main Article

Table 1

Virulence profiles and SBI types of Shiga toxin–producing Escherichia coli O157:H7 isolates obtained from humans and fecal samples from slaughterhouse cattle, New Zealand, 2008–2011*

Species, no. isolates NI SI Virulence genes†
 SBI type
ehxA eae stx2 stx2c stx1 Dominant (no., %) Other (no., %)
Bovine
6 6 0 + + + − + WY12a (6, 100.0) –
10 2 8 + + + + − ASY2c (7, 70.0), SY2c (2, 20.0) AS2c (1, 10.0)
24
 24
 0
 +
 +
 +
 −
 −
AY2a (22, 91.7)
 AWY2a (2, 8.3)
Human
51 43 9 + + + – + WY12a (49, 96.2) AWY12a (2, 3.8)
1 0 1 + + + – + WY12a (1, 100.0) –
94 54 40 + + + + − ASY2c (69, 73.4), SY2c (15, 16.0) SWY2c (3, 3.2), ASWY2c (2, 2.1), AS2c (2, 2.1), Y2c (2, 2.1), ASY2a2c (1, 1.1)
214 179 35 + + + − − AY2a (210, 98.1) AWY2a (4, 1.9)
3 2 1 + + + + + ASY12c (2, 66.7) AS12c (1, 33.3)

*NI, North Island of New Zealand; SBI, Shiga toxin–encoding bacteriophage insertion; SI, South Island of New Zealand; +, gene present; −, gene absent.
†ehxA gene encodes for enterohemolysin; eae gene encodes for intimin; stx2, primers for detection of this gene did not differentiate between subtypes of Shiga toxin type 2; stx2c gene encodes for Shiga toxin subtype 2c; stx1, primers for detection of this gene did not differentiate between subtypes of Shiga toxin type 1.

Main Article

1Preliminary results from this study were presented at the New Zealand Veterinary Association Conference; June 16–20, 2014, Hamilton, New Zealand.

Page created: November 18, 2014
Page updated: November 18, 2014
Page reviewed: November 18, 2014
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