Volume 20, Number 12—December 2014
Dispatch
Putative New West Nile Virus Lineage in Uranotaenia unguiculata Mosquitoes, Austria, 2013
Table 2
Sequence identities between the newly identified WNV strain from Austria, WNV-Uu-LN-AT-2013, and other strains representing different WNV lineages*
| Strain/lineage† |
Nucleotide identity or amino acid identity, %, by strain/lineage†‡ |
||||||||||
| WNV-Uu-LN-AT-2013 |
1a |
1b |
1c/5 |
2 |
3 |
4 |
6 (Spain)§ |
7 (Koutango virus) |
8¶ |
Usutu virus |
|
| WNV-Uu-LN-AT-2013 | 88.3 | 87.9 | 87.0 | 88.8 | 86.7 | 96.2 | 95.9 | 85.3 | 81.2 | 75.5 | |
| 1a | 76.2 | 97.6 | 93.4 | 94.0 | 90.4 | 88.6 | 91.7 | 89.2 | 92.4 | 76.3 | |
| 1b | 75.4 | 88.2 | 92.7 | 93.5 | 89.8 | 88.3 | 91.2 | 88.8 | 92.0 | 76.1 | |
| 1c/5 | 76.3 | 80.5 | 79.7 | 92.1 | 88.8 | 87.4 | 89.1 | 87.7 | 91.2 | 76.1 | |
| 2 | 77.0 | 79.8 | 79.6 | 79.1 | 90.9 | 89.2 | 92.6 | 89.3 | 92.0 | 76.0 | |
| 3 | 75.9 | 78.3 | 77.3 | 77.3 | 78.7 | 87.0 | 91.4 | 86.6 | 89.2 | 75.5 | |
| 4 | 82.8 | 76.6 | 76.0 | 76.2 | 76.9 | 76.5 | 95.0 | 85.5 | 81.0 | 74.7 | |
| 6 (Spain)§ | 83.2 | 78.1 | 78.1 | 77.7 | 78.6 | 79.5 | 81.7 | 88.6 | – | 80.8 | |
| 7 (Koutango virus) | 75.1 | 77.7 | 77.4 | 77.0 | 77.8 | 76.3 | 75.6 | 78.0 | 86.8 | 75.3 | |
| 8¶ | 72.7 | 78.4 | 78.0 | 77.3 | 78.4 | 77.7 | 72.6 | – | 77.4 | 76.3 | |
| Usutu virus | 71.2 | 72.4 | 72.6 | 72.4 | 71.3 | 71.0 | 70.1 | 73.6 | 72.4 | 72.5 | |
*Alignments were performed by using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/). WNV, West Nile virus; WNV-Uu-LN-AT-2013, West Nile virus strain Uranotaenia unguiculata-Lake Neusiedl-Austria-2013; –, comparison between lineages 6 and 8 was not possible because the available partial sequences do not cover the same nucleotide regions.
†GenBank accession nos. are as follows for the polyprotein genes/polyproteins: WNV-Uu-LN-AT-2013 (KJ831223), lineage 1a (AF404756/AAM81752), lineage 1b (D00246/BAA00176), lineage 1c (DQ256376/ABC40712), lineage 2 (DQ116961/AAZ91684), lineage 3 (AY765264/AAW81711), lineage 4 (FJ159129/ACH99530), lineage 6 (Spain) (GU047875/ADD69956), lineage 7 (Koutango virus) (EU082200/ABW76844), lineage 8 (KJ131502/AHV83443), Usutu virus (AY453411/AAS59402).
‡Amino acid sequences (above the diagonal) and nucleotide sequences (below the diagonal) are based on complete polyprotein genes, with the exception of lineage 6 and 8 strains, for which only partial sequences were available.
§Comparison was based only on partial NS5 gene sequences.
¶Comparison was based only on complete envelope protein gene sequences.
1Current affiliation: Paracelsus Medical University, Salzburg, Austria.