Volume 20, Number 12—December 2014
Novel Bluetongue Virus in Goats, Corsica, France, 2014
|PCR type||Target||Probe and primer names||Primer sequence, 5′ → 3′||Nucleotide location|
|Conventional BTV RT-PCR||Segment 2||PF||YRWTTGATTTTGARAARGA||1549–1566|
|Conventional RT-PCR||BTV-n segment 2||BTV-n F||CAGATCTGGTTTTACCGAG||1546–1564|
|Real-time RT-PCR||BTV-n segment 2||BTV-n F3||TGGATCATGATGGTTATGAACACC||1942–1966|
*BTV, bluetongue virus; RT, reverse transcription; PF, forward primer; PR, reverse primer.
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Page updated: November 19, 2014
Page reviewed: November 19, 2014
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