Volume 20, Number 12—December 2014
Health Care Worker Contact with MERS Patient, Saudi Arabia
To investigate potential transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) to health care workers in a hospital, we serologically tested hospital contacts of the index case-patient in Saudi Arabia, 4 months after his death. None of the 48 contacts showed evidence of MERS-CoV infection.
Middle East respiratory syndrome coronavirus (MERS-CoV) was initially isolated in September 2012 from a 60-year-old man from Bisha, Saudi Arabia (1). In June 2012, this index case-patient was hospitalized for severe respiratory disease in Jeddah at Dr. Soliman Fakeeh Hospital and subsequently died (1). As of November 3, 2014, a total of 897 laboratory-confirmed cases of MERS-CoV infection, including 325 deaths, had been reported to the World Health Organization; >85% of reported MERS-CoV cases and deaths have occurred in Saudi Arabia (2). The clinical syndrome among hospitalized MERS-CoV patients includes severe acute respiratory illness, sometimes associated with hypoxemic respiratory failure and extrapulmonary organ dysfunction (3); however, milder illness and asymptomatic infections have been identified through contact investigations (4–6). Transmission of MERS-CoV to health care workers (HCWs) has been reported (5,6), although no sustained community transmission has been identified.
A zoonotic origin of MERS-CoV has been hypothesized; camels potentially play a role in transmission (7), although the specific types of exposure associated with primary cases remain unknown. There remains a dearth of information on how MERS-CoV is spread and on transmission risks to HCW or other close contacts. Our objectives were to evaluate the degree and nature of HCW contact with the MERS-CoV index case-patient and to serologically assess HCWs for MERS-CoV infection. The field investigation was performed in October 2012; we awaited development and validation of MERS-CoV serologic assays before completing the study.
The index case-patient was hospitalized on June 13, 2012, with a 7-day history of fever, cough, sputum expectoration, and shortness of breath. Precautions to prevent airborne transmission were taken by placing the patient in a private room with negative pressure for the first 2 days of hospitalization. After an infectious disease consultation on day 2, airborne-transmission precautions were replaced with droplet-transmission precautions; after a multidrug-resistant organism was isolated on day 4, contact-transmission precautions were implemented (8). The case-patient remained in a private room, under standard and contact-transmission precautions, throughout hospitalization until he died on day 11.
Using dates and units in which the case-patient received care, hospital staff initially identified HCWs who had had contact with the case-patient (came within 2 meters of the case-patient or his bedding, equipment, or body fluids). A comparison group of approximately equal numbers of HCWs (preferentially with similar job responsibilities) was selected from HCW who had had no known contact with the case-patient. Hospital infection control staff administered a brief, standardized questionnaire to both groups of HCWs. Information was collected on HCW demographics, job duties, and symptoms of respiratory disease during June 15–July 4, 2012, which corresponds to the period when the case-patient was hospitalized and an incubation period of 2–10 days, based on MERS-CoV natural history information available at the time of investigation. Specific information about circumstances of case-patient contact and potential for MERS-CoV exposure was collected from HCWs who had had contact with the case-patient.
In October 2013 (4 months after the case-patient’s death), a blood specimen (<20 mL) was collected from each HCW and transported first to the Ministry of Health Western Regional Laboratory in Saudi Arabia and then to the US Centers for Disease Control and Prevention for MERS-CoV testing. Upon receipt, specimens were gamma-irradiated on dry ice and stored at −70°C. All specimens were tested by HKU5.2N nucleocapsid enzyme immunoassay (EIA) (9); incubations and substrate development were conducted at 37°C. MERS-CoV antibody positivity was defined as positive HKU5.2N screening EIA results and confirmed by MERS-CoV immunofluorescence or microneutralization assay (9,10); specimens with negative EIA results were considered antibody negative.
Of 56 HCWs identified as having had contact with the MERS-CoV case-patient, 5 were unavailable for interview and 3 refused serum collection, leaving 48 for the final analysis. Among HCW who had had case-patient contact, median age was 30.5 (range 22–57) years; 29 (60%) were female; 24 (50%) were nurses; 14 (29%) were physicians; 7 (15%) were respiratory technicians; and 1 each was a housekeeping, radiology, or infection control staff member. Six (13%) HCWs reported having a chronic medical condition (e.g., asthma, diabetes, hypertension), and 6 (13%) reported smoking tobacco. According to body mass index (BMI) calculations from self-reported height and weight, nearly half of HCWs were overweight (BMI 25.0–29.9, n = 11 [23%]) or obese (BMI ≥30.0, n = 12 [25%]).
Most of the 48 HCWs had reportedly come within 1 meter of the case-patient (89%); touched the case-patient (85%); or touched the case-patient’s bedding, equipment, or body fluids (62%) (Table). During a single shift, most (60%) HCWs reported <1 hour of case-patient contact, but 23% reported >5 hours of contact. HCWs reported having been present during airway suction (50%), nebulizer treatment (30%), sputum induction (23%), bronchoscopy (9%), and intubation (6%). Infection control precautions reportedly used by HCWs during contact with the case-patient included hand hygiene (100%) and/or wearing of gloves (94%), surgical mask (87%), and gown (40%); however, among those reporting use of these precautions, some admitted to <100% compliance and none used eye protection.
Respiratory disease symptoms during June 15–July 4 were reported by 10 (21%) HCWs who had had case-patient contact. Among these, symptoms included cough (40%), sore throat (30%), myalgia (30%), rhinorrhea (20%), self-reported fever (10%), diarrhea (10%), and sneezing (10%); 2 (20%) of these 10 HCWs sought medical care and received a diagnosis of pharyngitis. For comparison, respiratory symptoms were reported during the same period by 16 (33%) HCWs who had not had case-patient contact. Results of EIA testing of serum collected during October 14–17, 2012, from all 48 HCWs who had and all 48 who had not had case-patient contact were negative for MERS-CoV. Immunofluorescence assay of 13 randomly selected serum specimens also gave negative MERS-CoV results, supporting interpretation of EIA-negative specimens as antibody negative.
This investigation provides indicators of transmission potential during the initial emergence of MERS-CoV. The lack of MERS-CoV transmission among HCWs in this study is similar to results of some published contact investigations (11,12) but different from others that reported transmission to HCW contacts (5,6). Recovery of cultivable virus from the sputum of the case-patient reported here (1) and the severity of illness suggest some potential for virus transmission. We did not assess use of recommended personal protective equipment during each episode of case-patient contact; however, the reported lack of use during every episode of patient contact suggests that some HCWs might have been exposed to MERS-CoV. Nonetheless, the infection control precautions that were used might have contributed to the demonstrated lack of transmission.
Serologic assays to determine past infections are useful for assessing the risk for MERS-CoV transmission (13). The assays used in this study have previously detected MERS-CoV–specific antibodies <2 weeks and >13 months after illness onset with >97% specificity (9,14). Although no severely ill HCWs were identified during this investigation, real-time reverse transcription PCR detection of MERS-CoV in respiratory tract specimens from asymptomatic or mildly ill HCW during other contact investigations has been described (5,15). The timing of this investigation 4 months after hospitalization of the case-patient precluded use of real-time reverse transcription PCR and potentially introduced recall bias during HCW interviews.
Despite numerous case-patient contact episodes among HCWs, we found no serologic evidence suggesting health care–associated transmission of MERS-CoV from the index case-patient. Rapid identification of potentially infected patients and implementation of infection control precautions can help protect HCWs. US Centers for Disease Control and Prevention recommendations for management of hospitalized patients with known or suspected MERS-CoV infection include implementing standard, contact, and airborne transmission precautions (15).
Dr Hall is a veterinarian and an epidemiologist in the Division of Viral Diseases of the National Center for Immunization and Respiratory Diseases at the US Centers for Disease Control and Prevention. His research interests focus on the epidemiology of emerging viral diseases, specifically caliciviruses and coronaviruses.
We are extremely grateful for the collaboration of the participating HCW in this investigation. Additionally, we thank Idris Sulemann for assistance with data entry and management and Jennifer L. Harcourt, Hayat Caidi, and Azaibi Tamin for performing the serologic evaluations.
This work was supported by the US Global Disease Detection Operations Center Outbreak Response Contingency Fund.
- Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012;367:1814–20. DOIPubMedGoogle Scholar
- World Health Organization. Middle East respiratory syndrome coronavirus (MERS-CoV)— update. Disease Outbreak News. 2014 Nov 3 [cited 2014 Nov 4]. http://www.who.int/csr/don/2014_07_14_mers/en/
- Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, Al-Rabiah FA, Al-Hajjar S, Al-Barrak A, Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. Lancet Infect Dis. 2013;13:752–61. DOIPubMedGoogle Scholar
- Health Protection Agency UK Novel Coronavirus Investigation Team. Evidence of person-to-person transmission within a family cluster of novel coronavirus infections, United Kingdom, February 2013. Euro surveillance. 2013;18: pii: 20427.
- Assiri A, McGeer A, Perl TM, Price CS, Al Rabeeah AA, Cummings DA, Hospital outbreak of Middle East respiratory syndrome coronavirus. N Engl J Med. 2013;369:407–16. DOIPubMedGoogle Scholar
- Memish ZA, Zumla AI, Assiri A. Middle East respiratory syndrome coronavirus infections in health care workers. N Engl J Med. 2013;369:884–6. DOIPubMedGoogle Scholar
- Memish ZA, Cotten M, Meyer B, Watson SJ, Alsahafi AJ, Al Rabeeah AA, Human infection with MERS coronavirus after exposure to infected camels, Saudi Arabia, 2013. Emerg Infect Dis. 2014;20:1012–5 and. DOIPubMedGoogle Scholar
- Centers for Disease Control and Prevention. 2007 Guideline for isolation precautions: preventing transmission of infectious agents in healthcare settings [cited 2014 Jun 30]. http://www.cdc.gov/hicpac/2007IP/2007isolationPrecautions.html
- Al-Abdallat MM, Payne DC, Alqasrawi S, Rha B, Tohme RA, Abedi GR, Hospital-associated outbreak of Middle East respiratory syndrome coronavirus: a serologic, epidemiologic, and clinical description. Clin Infect Dis. 2014; May 14. pii: ciu359 [cited 2014 Jul 8]. [Epub ahead of print].
- Payne DC, Iblan I, Alqasrawi S, Al Nsour M, Rha B, Tohme RA, Stillbirth during infection with Middle East respiratory syndrome coronavirus. J Infect Dis. 2014;209:1870–2. DOIPubMedGoogle Scholar
- Pebody RG, Chand MA, Thomas HL, Green HK, Boddington NL, Carvalho C, The United Kingdom public health response to an imported laboratory-confirmed case of a novel coronavirus in September 2012. Euro Surveill. 2012;17:20292.PubMedGoogle Scholar
- Mailles A, Blanckaert K, Chaud P, van der Werf S, Lina B, Caro V, First cases of Middle East respiratory syndrome coronavirus (MERS-CoV) infections in France, investigations and implications for the prevention of human-to-human transmission, France, May 2013. Euro Surveill. 2013;18:20502.PubMedGoogle Scholar
- Consortium for the Standardization of Influenza Seroepidemiology. Novel coronavirus (MERS-CoV)[cited 2014 Aug 12]. https://consise.tghn.org/articles/novel-coronavirus-ncov/
- Kapoor M, Pringle K, Kumar A, Dearth S, Liu L, Lovchik J, Clinical and laboratory findings of the first imported case of Middle East respiratory syndrome coronavirus (MERS-CoV) into the United States. Clin Infect Dis. 2014 Aug 6. pii: ciu635 [cited 2014 Aug 6]. [Epub ahead of print].
- Bialek SR, Allen D, Alvarado-Ramy F, Arthur R, Balajee A, Bell D, First confirmed cases of Middle East respiratory syndrome coronavirus (MERS-CoV) infection in the United States, updated information on the epidemiology of MERS-CoV infection, and guidance for the public, clinicians, and public health authorities—May 2014. MMWR Morb Mortal Wkly Rep. 2014;63:431–6.PubMedGoogle Scholar
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Table of Contents – Volume 20, Number 12—December 2014
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Please use the form below to submit correspondence to the authors or contact them at the following address:
Aron J. Hall, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop A34, Atlanta, GA 30329-4027, USA; ; Ziad Memish, Ministry of Health, Riyadh 11176, Saudi Arabia