Volume 20, Number 5—May 2014
Letter
Extended-Spectrum β-Lactamases in Escherichia coli and Klebsiella pneumoniae in Gulls, Alaska, USA
Table
Characterization of Escherichia coli and Klebsiella pneumoniae ESBL-producing isolates, Barrow, Alaska, USA*
| Isolates, no.† | bla genotype |
MLST profile | |||
|---|---|---|---|---|---|
| CTX-M | SHV | TEM | |||
| E. coli | |||||
| 12 | 14 | – | 1 | ST38 (ST2253)‡ | |
| 11 | 14 | – | – | ST131 (ST10)§ | |
| 5 | – | – | 19 | ST2967¶ | |
| 3 | 27 | – | – | ST405 | |
| 1 | 15 | – | – | ST131 | |
| 1 | – | – | 1 | ST2967¶ | |
| K. pneumoniae | |||||
| 4 | 15 | 12 | 1 | ND | |
| 5 | – | 12 | 1 | ND | |
| 2 | – | 12 | – | ND | |
| 2 | – | 102 | 19 | ND | |
| 8 | – | 102 | – | ND | |
| 1 | – | – | 19 | ND | |
| 4 | 15 | 1 | 1 | ND | |
| 1 | – | 2 | – | ND | |
*ESBL, extended-spectrum β-lactamase; MLST, multilocus sequence type; ST, sequence type; ND, no data.
†E. coli comprised 33 isolates from 32 samples. K. pneumoniae comprised 35 isolates from 35 samples. and 12 samples were both E. coli and K. pneumoniae ESBL-harboring isolates but did not display horizontal transfer resulting from deviating resistance genotypes.
‡One of the isolates harbored a novel MLST allele, giving the novel ST2253 (deposited in the E. coli MLST database at the ERI, University College, Cork, Ireland, UK (http://mlst.ucc.ie/mlst/mlst/dbs/Ecoli/).
§One of the isolates had ST10; the remaining 10 had ST131.
¶Isolates harbored a novel MLST allele, rendering the novel ST2967 (deposited in the MLST database). The single isolate with only blaTEM-1 may contain undetected ESBL genes because of the non-ESBL phenotype of TEM-1.