TY - JOUR AU - Wang, Wei AU - Song, Zhigang AU - Guan, Wencai AU - Liu, Yi AU - Zhang, Xiaonan AU - Xu, Lei AU - Li, Jianhua AU - Yuan, Zhenghong AU - Hu, Yunwen T1 - PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus T2 - Emerging Infectious Disease journal PY - 2014 VL - 20 IS - 5 SP - 847 SN - 1080-6059 AB - Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens. KW - influenza KW - influenza virus KW - viruses KW - H7N9 KW - influenza A(H7N9) virus KW - neuraminidase KW - R292K KW - single-nucleotide polymorphism KW - SNP KW - real-time reverse transciption PCR KW - real-time RT-PCR KW - oseltamivir resistance KW - mutation KW - NA 292K mutant virus KW - R292K wild-type virus KW - real-time reverse transcription PCR DO - 10.3201/eid2005.131364 UR - https://wwwnc.cdc.gov/eid/article/20/5/13-1364_article ER - End of Reference