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Volume 20, Number 6—June 2014
Research

Genetic Evidence of Importation of Drug-Resistant Plasmodium falciparum to Guatemala from the Democratic Republic of the Congo

Jaymin C. PatelComments to Author , Steve M. Taylor, Patricia C. Juliao, Christian M. Parobek, Mark Janko, Luis Demetrio Gonzalez, Lucia Ortiz, Norma Padilla, Antoinette K. Tshefu, Michael Emch, Venkatachalam Udhayakumar, Kim Lindblade, and Steven R. Meshnick
Author affiliations: University of North Carolina, Chapel Hill, North Carolina, USA (J.C. Patel, S.M. Taylor, C.M. Parobek, M. Janko, M. Emch, S.R. Meshnick); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (P.C. Juliao, V. Udhayakumar, K. Lindblade); Military Medical Center, Guatemala City, Guatemala (L.D. Gonzalez); Universidad de Valle de Guatemala, Guatemala City (L. Ortiz, N. Padilla); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A.K. Tshefu)

Main Article

Table

Neutral microsatellite loci and primer sequences used for PCR amplification of Plasmodium falciparum malaria genes to identify origin of drug-resistant genotype, Guatemala

Locus name
 Chromosome
 Primer
 Primer sequence (5′→3′)
 Tag
 Expected product size, bp
C2M33 2 Forward CATTGCAAAATATATATTCTCC FAM 193
Reverse GTGATTTGTACAATGTACATA
Heminested
 ATTGCGTAAATAACACATCTGCA
C3M88* 3 Forward CAAAAATGAAAAATGAAAAGG HEX 150
Reverse TAAAGGGTGCGCATATCAAT
Heminested
 GTTATTCAAAAAGGACGAAACAAG
C3M69 3 Forward AATAGGAACAAATCATATTG HEX 173
Reverse AGATATCCAGGTAATAAAAAG
Heminested
 TTTATGAACACCCTCATGTCACT
C2M29 2 Forward GTGAATAACGGAAAAGGATA FAM 141
Reverse AAGATCAAATACCAGGTGA
Heminested
 TTAAGAAACAATCAGAAGCGATG
C2M27 2 Forward CTTTTAATCACTACCATGTTG HEX 117
Reverse ATAATTTAATTGAGGATACCT
Heminested
 TTGTATGTATCACTTTTTCATTAC
C3M40 3 Forward GGGTAAAGAAAAACACACAAA FAM 128
Reverse AATGTGTATATTACTAGAAGC
Heminested
 TCCGAATATGGAATGTCGAAAG
C3M39 3 Forward CAAGAAGATAGGGATGATAAC FAM 159
Reverse TATTAATTGGTCTTCACCCG
Heminested
 GGAGGAACGTAAAGAAGATATTG
C2M34 2 Forward TCCCTTTTAAAATAGAAGAAA FAM 260
Reverse GATTATATGAAAGGATACATG
Heminested TTCACTTTGTAAATTAGAACATATC

*Censored locus caused by poor amplification success.

Main Article

Page created: May 16, 2014
Page updated: May 16, 2014
Page reviewed: May 16, 2014
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