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Volume 20, Number 6—June 2014

Fatal Monkeypox in Wild-Living Sooty Mangabey, Côte d’Ivoire, 2012

Aleksandar Radonić, Sonja Metzger, Piotr Wojtek Dabrowski, Emmanuel Couacy-Hymann, Livia Schuenadel, Andreas Kurth, Kerstin Mätz-Rensing, Christophe Boesch, Fabian H. Leendertz, and Andreas NitscheComments to Author 
Author affiliations: Robert Koch Institute, Berlin, Germany (A. Radonić, S. Metzger, P.W. Dabrowski, L. Schuenadel, A. Kurth, F.H. Leendertz, A. Nitsche); Laboratoire de la Pathologie Animale, Bingerville, Côte d’Ivoire (E. Couacy-Hymann); Deutsches Primatenzentrum, Göttingen, Germany (K. Mätz-Rensing); Max-Planck-Institute for Evolutionary Anthropology, Leipzig, Germany (C. Boesch)

Main Article


Results of quantitative PCR from tissues of a wild-living sooty mangabey (Cercocebus atys), Taï National Park, Côte d’Ivoire, March 2012*

Tissue Ct OPV rpo18 Ct c-myc ΔCt, rpo18–c-myc
Spleen 32.0 22.1 –9.9
Lung 34.3 27.8 –6.5
Kidney 28.2 18.9 –9.3†
Skin 16.9 26.9 9.9
Liver 30.3 20.0 –10.3†
Heart 32.2 25.0 –7.2
Intestine 32.2 31.1 0.1
Muscle ND ND ND
Thymus 15.8 19.7 3.9†
Throat swab 21.5 28.4 6.9†
Lymph node 24.1 20.2 –3.9†

*Virus DNA (rpo18) was quantified in relation to cellular c-myc DNA in 1 μL DNA; higher values indicate higher virus loads in a respective tissue. Ct, cycle threshold; OPV, orthopoxvirus; ND, OPV DNA not detectable.
†5 μL used in PCR.

Main Article

Page created: May 16, 2014
Page updated: May 16, 2014
Page reviewed: May 16, 2014
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