Novel Human Bufavirus Genotype 3 in Children with Severe Diarrhea, Bhutan
Takaaki Yahiro, Sonam Wangchuk, Kinlay Tshering, Purushotam Bandhari, Sangay Zangmo, Tshering Dorji, Karchung Tshering, Takashi Matsumoto, Akira Nishizono, Maria Söderlund-Venermo, and Kamruddin Ahmed
Author affiliations: Faculty of Medicine, Oita University, Yufu, Japan (T. Yahiro, T. Matsumoto, A. Nishizono, K. Ahmed); Ministry of Health, Thimphu, Bhutan (S. Wangchuk, S. Zangmo, T. Dorji, K. Tshering); Jigme Dorji Wangchuk National Referral Hospital, Thimphu (K. Tshering); Mongar Regional Referral Hospital, Mongar, Bhutan (P. Bandhari); University of Helsinki, Helsinki, Finland (M. Söderlund-Venermo)
Figure. Phylogenetic trees of the viral protein 1 (VP1) of bufaviruses, constructed by using deduced amino acid sequences by neighbor-joining methodThe full length open reading frames of VP1 genes were used to deduce amino acid sequencesPorcine parvovirus strain NC 001718 was used as an out-groupThe numbers adjacent to the nodes represent the bootstrap values, and values <50% are not shownScale bar indicates the genetic distances expressed as amino acid substitutions per siteAccession numbers for DNA Data Bank of Japan, European Molecular Biology Laboratory, and GenBank are AB847987, AB847988, and AB847989 for strains BTN-63, BTN-109, and BTN-310, respectively.
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