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Volume 20, Number 7—July 2014
Dispatch

Diversity of Francisella tularensis Subsp. holarctica Lineages, China

Yanhua Wang, Yao Peng, Rong HaiComments to Author , Lianxu Xia, Hang Li, Zhikai Zhang, Hong Cai, Ying Liang, Xiaona Shen, Dongzheng Yu, Dawn Birdsell, David M. Wagner, and Paul KeimComments to Author 
Author affiliations: National Institute for Communicable Disease Control and Prevention; State Key Laboratory for Infectious Disease Prevention and Control; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China (Y. Wang, Y. Peng, R. Hai, L. Xia, Z. Zhang, H. Cai, Y. Liang, X. Shen, D. Yu); Beijing Genomics Institute–Shenzhen, Shenzhen, China (H. Li); Northern Arizona University, Flagstaff, Arizona, USA (D. Birdsell, D.M. Wagner, P. Keim)

Main Article

Table 2

Primers used in sequencing to obtain canonical SNP loci for Francisella tularensis subspholarctica isolates*

SNP SCHU S4 SNP position† SNP state (D/A)‡ Primer sequence, 5′ → 3′ Annealing temperature, °C§
F.3 910179 G/A F: GCTGTATCATCATTTAATAAACTGCTG 55
R: TTGGGAAGCTTGTATCATGGCACT
B.2 5162 A/C F: TTAGTCTATGAGCAGCCAG 50
R: TAATATCACCAAGGTAGCC
B.3 470841 A/G F: ACGCTAGGTGTCTTGGT 50
R: CTATATCCGCTCAACAT
B.4 823672 T/A F: TAGACGCACTGGATTTAGGT 53.5
R: AACCATCACGCCACCATAAG
B.5 1853655 T/C F: TGGATCAAACAACCGT 50
R: TCTCAAGAGCTGGTGC
B.6 713647 A/G F: AGTAGTGGTAGCGAGGC 53.5
R: TACCGTTAGCCCAACAG
B.12 109781 T/A F: TACTGCCCAACATAGAG 55
R: ATCGTGATAAGGCTGGA
B.16 608245 T/G F: ATGCTAGCAAATTACCATCAAAAG 57
R: AACTCTTCTCGCCATCAACTTCTAT
B.17 1743207 A/C F: CCAAGAGCTAAATTAGCTTCAA 53.5
R: TGACCAAGAAGGTAGAGGTATTGGTT
B.19 1373999 A/C F: TTGCTACTGATGGTTTAACT 57
R: CAATACGTCACTTATGCAGTGAT
B.20 1396082, 1789417 C/T F: ATGGGTCGGACTATCACATC 56
R: ATTATTGTTAAACGGCATCG
B.23 253120 A/C F: GGCAACAGCAGATTCGTGAG 56
R: TGAAAGCAGGTTTAGAAGGACAG

*SNP, single-nucleotide polymorphism; D, derived; A, ancestral; F, forward; R, reverse.
†SNP position based on the reference isolate Schu S4 (NC_006570).
‡Top strand orientation of SCHU S4.
§Sequencing conditions as described in (10).

Main Article

Page created: June 17, 2014
Page updated: June 17, 2014
Page reviewed: June 17, 2014
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