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Volume 20, Number 8—August 2014
Dispatch

Human Infections with Borrelia miyamotoi, Japan

Kozue Sato, Ai Takano, Satoru Konnai, Minoru Nakao, Takuya Ito, Kojiro Koyama, Minoru Kaneko, Makoto Ohnishi, and Hiroki KawabataComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (K. Sato, M. Ohnishi, H. Kawabata); Yamaguchi University, Yamaguchi, Japan (A. Takano); Hokkaido University, Sapporo, Japan (S. Konnai); Asahikawa Medical College, Asahikawa, Japan (M. Nakao); Hokkaido Institute of Public Health, Sapparo (T. Ito); Oumu National Health Insurance Hospital, Oumu, Japan (K. Koyama); Kamifurano Hospital, Kamifurano, Japan (M. Kaneko, Japan); Gifu University, Gifu, Japan (H. Kawabata)

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Figure

Immunoblot analysis of serum reactivity to antigens of Borrelia miyamotoi and Lyme disease borreliae, Japan. Serum samples obtained from 2 patients were examined. For case-patient 1, acute-phase serum obtained on July 25, 2011, and convalescent-phase serum obtained on August 10 were used. For case-patient 2, acute-phase serum obtained on June 11 was used. A) Reactivity to recombinant glycerophosphodiester phosphodiesterase (GlpQ) antigen. Crude rGlpQ were used for immunoblot analysis (10). Recom

Figure. Immunoblot analysis of serum reactivity to antigens of Borrelia miyamotoi and Lyme disease borreliae, JapanSerum samples obtained from 2 patients were examinedFor case-patient 1, acute-phase serum obtained on July 25, 2011, and convalescent-phase serum obtained on August 10 were usedFor case-patient 2, acute-phase serum obtained on June 11 was usedA) Reactivity to recombinant glycerophosphodiester phosphodiesterase (GlpQ) antigenCrude rGlpQ were used for immunoblot analysis (10)Recombinant GlpQ was separated by electrophoresis on a 5%–20% polyacrylamide gradient gel (Wako Pure Chemical Industries Inc., Osaka, Japan), and antigen was stained with Coomassie brilliant blueCBB, protein profileMolecular mass markers are shown on the leftB) Reactivity of patient serum samples to whole cell lysate of Bmiyamotoi antigensA low-passage strain of Bmiyamotoi (strain MYK3) was used for immunoblot analysis (10)A negative control was serum obtained from a healthy human (resident of an area to which Lyme disease was not endemic)Molecular mass markers are shown on the leftC) Serodiagnosis of Lyme disease by immunoblot analysis of serum samples from the 2 patientsOspC, outer surface protein C.

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