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Volume 20, Number 8—August 2014

New Introductions of Enterovirus 71 Subgenogroup C4 Strains, France, 2012

Isabelle SchuffeneckerComments to Author , Cécile Henquell, Audrey Mirand, Marianne Coste-Burel, Stéphanie Marque-Juillet, Delphine Desbois, Gisèle Lagathu, Laure Bornebusch, Jean-Luc Bailly, and Bruno Lina
Author affiliations: Hospices Civils de Lyon, Lyon, France (I. Schuffenecker, B. Lina); Centre Hospitalier Universitaire de Clermont-Ferrand, Clermont-Ferrand, France (C. Henquell, A. Mirand); Université d’Auvergne, Clermont-Ferrand (C. Henquell, A. Mirand, J.-L. Bailly); Centre Hospitalier Universitaire de Nantes, Nantes, France (M. Coste-Burel); Centre Hospitalier de Versailles, Le Chesnay, France (S. Marque-Juillet); Centre Hospitalier de Rambouillet, Rambouillet, France (D. Desbois); Centre Hospitalier Universitaire de Rennes, Rennes, France (G. Lagathu); Centre Hospitalier de Grasse, Grasse, France (L. Bornebusch)

Main Article


Characteristics of 4 patients with enterovirus 71 subgenogroup C4 infections, France, 2012*

Patient no., hospital location† Date admitted Age at diagnosis Clinical signs Laboratory values for CSF
Enterovirus RT-PCR results, Ct
Duration, d
Leukocytes/ mm3 Protein, g/L CSF NP Hospital stay Antimicrobial drug use
1, Nantes Feb 12 6 d Fever syndrome <2 0.54 Pos, 36.0 Pos, 36.2 4.0 4.0
2, Versailles Mar 12 17 d Fever syndrome 53 0.52 Pos, 31.7 NA 2.5 2.5
3, Grasse Jun 12 4.5 y Fever syndrome, meningitis 83§ 0.92§ Neg Pos, 27.4 1.0 0
4, Rennes Oct 12 21 d Fever syndrome, rhinitis 234 0.77 Pos, 30.0 NA 6.0 3.0

*For all 4 children, molecular testing results were negative for the detection of herpes simplex virus types 1 and 2 and varicella-zoster virus in CSF. In addition, for patients 1 and 4, molecular testing results were also negative for the detection of human herpesvirus type 6, cytomegalovirus, and Epstein-Barr virus. Results were also negative for CSF bacterial cultures. CSF, cerebrospinal fluid sample; Ct, cycle threshold; NA, not available; Neg, negative NP, nasopharyngeal sample; Pos, positive; RT-PCR, reverse transcription PCR.
†Nantes (47°13′N; 1°33′W; Brittany); Versailles (48°48′N; 2°08′E; Ile de France); Grasse (43°40′N; 6°55′E; Provence-Alpes-Côte d’Azur); Rennes (48°06′N; 1°40′W; Brittany).
‡The upper Ct for positivity for each of the RT-PCR assays used was 45.0. An in-house enterovirus real-time RT-PCR was used to test samples from patient 1. A commercial RT-PCR assay produced by Cepheid and commercialized by Orgentec (GP-EV-040, Orgentec, Trappes, France) was used to test samples from patients 2 and 3. Another commercial RT-PCR assay, Dia-ENT-050 (Diagenode, Seraing, Belgium) was used to test samples from patient 4.
§CSF sample was hemorrhagic (10,300 erythrocytes/mm3).

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Page created: July 18, 2014
Page updated: July 18, 2014
Page reviewed: July 18, 2014
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