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Volume 21, Number 10—October 2015
Dispatch

Detection of Mixed Infections with Plasmodium spp. by PCR, India, 2014

Sri Krishna, Praveen K. Bharti, Himashu S. Chandel, Amreen Ahmad, Rajesh Kumar, Puspendra P. Singh, Mrigendra P. Singh, and Neeru SinghComments to Author 
Author affiliations: National Institute for Research in Tribal Health, Jabalpur, India (S. Krishna, P.K. Bharti, H.S. Chandel, A. Ahmad, R. Kumar, P.P. Singh, N. Singh); National Institute for Malaria Research Field Unit, Jabalpur (M.P. Singh)

Main Article

Table 2

Characteristics of PCR primers specific for 18S rRNA gene of Plasmodium spp., India*

Genus or species
 Primer
 Sequence, 5′→3′
 PCR product, bp
 PCR Program No. cycles
Denaturation
 Annealing
 Elongation
Temp, °C
 Time, min
 Temp, °C
 Time, min
 Temp ,°C
 Time, min
Plasmodium F TTAAAATTGTTGCAGTTAAAACG 1,200 94 1 58 2 72 2 25
R CCTGTTGTTGCCTTAAACTTC 1,200 94 1 58 2 72 2 25
P. falciparum F TTAAACTGGTTTGGGAAAACCAAATATATT 205 94 1 58 2 72 2 30
R ACACAATGAACTCAATCATGACTACCCGTC 205 94 1 58 2 72 2 30
P. vivax F CGCTTCTAGCTTAATCCACATAACTGATAC 120 94 1 58 2 72 2 30
R ACTTCCAAGCCGAAGCAAAGAAAG TCCTTA 120 94 1 58 2 72 2 30
P. malariae F ATAACATAGTTGTACGTTAAGAATAACCGC 144 94 1 58 2 72 2 30
R AAAATTCCCATGCATAAAAAATTATACAAA 144 94 1 58 2 72 2 30
P. ovale F ATCTCTTTTGCTATTTTTTAGTATTGGAGA 800 94 1 58 2 72 2 30
R GGAAAAGGACACATTAATTGTATCCTAGTG 800 94 1 58 2 72 2 30
P. knowlesi F CAGAGATCCGTTCTCATGATTTCCATGG 209 95 0.5 57 0.5 72 0.75 35
R CTRAACACCTCATGTCGTGGTAG 209 95 0.5 57 0.5 72 0.75 35
P. ovale F CTACTTGACATTTCTACTTACA 938 95 1 50 1 72 1 35
R CGTTCTTGATTAATGGAAGTAT 938 95 1 50 1 72 1 35
P. ovale (curtisi and wallikeri) F GCTGTAGCTAATACTTGCTTTA 827 95 1 55 1 72 1 25
R TTCACCTCTGACATCTGAATC 827 95 1 55 1 72 1 25

*F, forward; R, reverse.

Main Article

Page created: September 22, 2015
Page updated: September 22, 2015
Page reviewed: September 22, 2015
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