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Volume 21, Number 11—November 2015
Dispatch

RmtC and RmtF 16S rRNA Methyltransferase in NDM-1–Producing Pseudomonas aeruginosa

Mohibur Rahman, Kashi Nath PrasadComments to Author , Ashutosh Pathak, Binod Kumar Pati, Avinash Singh, Cristina M. Ovejero, Saheem Ahmad, and Bruno Gonzalez-Zorn
Author affiliations: Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India (M. Rahman, K.N. Prasad, A. Pathak, B.K. Pati, A. Singh); Integral University, Lucknow (M. Rahman, S. Ahmad); Complutense University of Madrid, Madrid, Spain (C.M. Overjero, B. Gonzalez-Zorn)

Main Article

Figure 2

A) Pulsed-field gel electrophoresis patterns of rmtC-positive Pseudomonas aeruginosa. Lane 1, λ ladder; 2, KnPa1A; 3, KnPa1B; 4, KnPa1C. B) Chromosomal location of rmtC, rmtF, and blaNDM-1 genes by I-CeuI-digested genomic DNA of P. aeruginosa isolates. Lane 5, KnPa1A; 6, KnPa1B; 7, KnPa1C; smears show Southern blot analysis of genomic DNA with probes specific to 16S rRNA, RmtC, RmtF, and NDM-1 genes.

Figure 2. A) Pulsed-field gel electrophoresis patterns of rmtC-positive Pseudomonas aeruginosa. Lane 1, λ ladder; 2, KnPa1A; 3, KnPa1B; 4, KnPa1C. B) Chromosomal location of rmtC, rmtF, and blaNDM-1 genes by I-CeuI-digested genomic DNA of P. aeruginosa isolates. Lane 5, KnPa1A; 6, KnPa1B; 7, KnPa1C; smears show Southern blot analysis of genomic DNA with probes specific to 16S rRNA, RmtC, RmtF, and NDM-1 genes.

Main Article

Page created: October 19, 2015
Page updated: October 19, 2015
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