Figure 1. Phylogenetic analysis segment sequences of Sochi virus, Russia: A) 347-bp large (L) segment sequence; B) 1,197-bp small (S) segment sequence. Virus sequences derived from patients (shown in bold type) and Apodemus ponticus mice cluster within the Sochi genotype of DOBV. Evolutionary analysis was conducted in MEGA6 (6). The evolutionary history was inferred by using the maximum-likelihood method based on the Tamura 3-parameter model with a discrete gamma distribution and 5 rate categories (analysis in panel A) and on the general time reversible model with gamma rates and heterogeneous patterns (analysis in panel B), respectively, which were estimated to be the best-fit substitution model according to the Bayesian information criterion. Scale bars indicate an evolutionary distance of 0.1 substitutions per position in the sequence. Bootstrap values >70%, calculated from 500 replicates, are shown at the tree branches. GenBank accession numbers of all sequences used in the analysis are listed in Technical Appendix Table 1). Dark gray shading iindicates cluster of DOBV-Sochi strains; light gray shading indicates different clusters of strains from other DOBV genotypes. ANDV, Andes virus; DOBV, Dobrava-Belgrade virus; HTNV, Hantaan virus; PUUV, Puumala virus; SANGV, Sangassou virus; SEOV, Seoul virus; SNV, Sin Nombre virus; THAIV, Thailand virus; TULV, Tula virus.