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Volume 21, Number 2—February 2015
Research

Evidence for Elizabethkingia anophelis Transmission from Mother to Infant, Hong Kong

Susanna K.P. Lau1, Alan K.L. Wu1, Jade L.L. Teng1, Herman Tse1, Shirly O.T. Curreem, Stephen K.W. Tsui, Yi Huang, Jonathan H.K. Chen, Rodney A. Lee, Patrick C.Y. Woo, and Kwok-Yung YuenComments to Author 
Author affiliations: The University of Hong Kong, Hong Kong (S.K.P. Lau, J.L.L. Teng, H. Tse, S.O.T. Curreem, Y. Huang, J.H.K. Chen, K.-Y. Yuen, P.C.Y. Woo); State Key Laboratory of Emerging Infectious Diseases, Research Centre of Infection and Immunology, Carol Yu Centre for Infection, Hong Kong (S.K.P. Lau, H. Tse, K.Y. Yuen, P.C.Y. Woo); Pamela Youde Nethersole Eastern Hospital, Hong Kong (A.K.L. Wu, R.A. Lee); School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong (S.K.W. Tsui)

Main Article

Figure 4

Pulsed-field gel electrophoresis (PFGE) analysis of samples from patients in Hong Kong showing 3 Elizabethkingia anophelis strains compared with reference Elizabethkingia isolates. A) PFGE performed by using CHEF Mapper XA system (Bio-Rad, Hercules, CA, USA) and restriction endonuclease XbaI shows that isolates from patient 2 and patient 3 are indistinguishable, wheras isolates from patient 1 possess distinct PFGE patterns. Lane 1, E. anophelis strain HKU37 from uterine swab specimen of patient

Figure 4. Pulsed-field gel electrophoresis (PFGE) analysis of samples from patients in Hong Kong showing 3 Elizabethkingia anophelis strains compared with reference Elizabethkingia isolates. A) PFGE performed by using CHEF Mapper XA system (Bio-Rad, Hercules, CA, USA) and restriction endonuclease XbaI shows that isolates from patient 2 and patient 3 are indistinguishable, wheras isolates from patient 1 possess distinct PFGE patterns. Lane 1, E. anophelis strain HKU37 from uterine swab specimen of patient 2; lane 2, placental swab specimen from patient 2; lane 3, E. anophelis strain HKU38 from blood of patient 3; lane 4, cerebrospinal fluid from patient 3; lane 5, E. anophelis strain HKU36 from blood of patient 1; lane 6, cerebrospinal fluid from patient 1; lane 7, E. anophelis type strain R26T; lane 8, E. meningoseptica type strain ATCC 13253T; lane 9, E. miricola type strain LMG22470T. B) Dendrogram constructed with PFGE data by similarity and clustering analysis using the Dice coefficient (1% tolerance and 0.5% optimization) and the unweighted pair-group method using average linkages with GelCompar II (Applied Maths, Sint-Martens-Latem, Belgium).

Main Article

1These authors contributed equally to this article.

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