Volume 21, Number 2—February 2015
Research
Novel Reassortant Influenza A(H5N8) Viruses among Inoculated Domestic and Wild Ducks, South Korea, 2014
Table 3
Virus titers in tissues of domestic ducks and mallard ducks inoculated intranasally with H5N8 and H5N1 influenza viruses
| Host species, virus |
No. ducks, mode of death* |
dpi |
Virus isolation titer in tissue samples (no. positive/no. ducks)† | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tra |
Liv |
Mus |
Pro |
Int, Pan |
Spl |
Ct |
Lung |
Kid |
Heart |
Brain |
|||
| Domestic ducks | |||||||||||||
| A/breeder duck/Kr/Gochang1/2014 (H5N8) |
3, euthanized | 3 | 6.7 ± 1.1 (3/3) | 1.5 ± 0.4 (2/3) | 1.0 ± 0.6 (2/3) | 2.6 ± 1.6 (3/3) | 0.9 ± 0.4 (2/3) | 2.4 ± 0.9 (3/3) | 2.8 ± 0.5 (2/3) | 4.2 ± 0.5 (3/3) | 3.2 ± 0.7 (3/3) | 2.4 ± 0.6 (3/3) | 1.6 ± 1.3 (2/3) |
| 1, died |
10 |
3.8 |
0 |
0 |
2.5 |
1.5 |
0 |
2.4 |
3.9 |
0 |
3.5 |
4.2 |
|
| A/broiler duck/Kr/Buan2/ 2014 (H5N8) |
3, euthanized |
3 |
4.7 ± 1.3 (3/3) |
2.2 ± 0.4 (3/3) |
1.9 ± 1.3 (3/3) |
2.8 ± 0.5 (3/3) |
0 (0/3) |
3.3 ± 0.6‡ (2/3) |
2.0 ± 0.8 (2/3) |
3.5 ± 0.1 (3/3) |
2.3 ± 0.9 (3/3) |
2.2 ± 0.6 (3/3) |
0 (0/3) |
| A/Baikal teal/Kr/Donglim3/ 2014 (H5N8) |
3, euthanized | 3 | 7.6 ± 0.9‡ (3/3) | 2.4 ± 1.2 (2/3) | 1.9 ± 0.7 (2/3) | 1.9 ± 0.9 (3/3) | 1.8 (1/3) | 0.7 ± 0.1 (2/3) | 2.3 ± 0.1 (2/3) | 4.5 ± 0.1‡ (3/3) | 2.8 ± 0.8 (3/3) | 1.6 ± 0.8 (3/3) | 4.0 (1/3) |
| 1, died |
10 |
0 |
0 |
0 |
0 |
1.5 |
1.5 |
0 |
0 |
0 |
0 |
0 |
|
| Control | |||||||||||||
| NI |
2, euthanized |
3 |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
| Mallard ducks | |||||||||||||
| A/broiler duck/Kr/Buan2/ 2014 (H5N8) |
2, euthanized |
3 |
2.6 (1/2) |
0 (0/2) |
2.2 (1/2) |
2.2 ± 1.9 (2/2) |
2.2 ± 0.5 (2/2) |
0 (0/2) |
1.8 (1/2) |
1.9 ± 0.7 (2/2) |
2.5 ± 1.8 (2/2) |
2.2 (1/2) |
0 (0/2) |
| A/chicken/Kr/IS/ 2006 (H5N1) |
2, euthanized |
3 |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
0 (0/2) |
| A/mandarin duck/Kr/PSC24–24/2010 (H5N1) | 2, euthanized | 3 | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) | 0 (0/2) |
*Tissues were collected from euthanized birds or birds that died from the virus on 3 dpi. dpi, days post inoculation; log10 TCID50, tissue culture infective dose; Tra, trachea; Liv, liver; Mus, muscle; Pro, proventriculus; Int, intestine; Pan, pancreas; Spl, spleen; Ct, cecal tonsil; Kid, kidney, NI, control ducks were not inoculated.
†Virus titers in tissues from domestic ducks inoculated intranasally with 106.5 50% egg-infective dose of H5N8 influenza virus; values are mean+SD TCID titers/0.1 mL.
‡Donglim3 viral titers were significantly higher than those of Buan2 virus from trachea and lung (p<0.05), whereas Buan2 viral titers from spleen were significantly higher than those of Donglim3 (p<0.05).