TY - JOUR AU - Zhao, Jiangqin AU - Ragupathy, Viswanath AU - Liu, Jikun AU - Wang, Xue AU - Vemula, Sai Vikram AU - El Mubarak, Haja Sittana AU - Ye, Zhiping AU - Landry, Marie AU - Hewlett, Indira T1 - Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses T2 - Emerging Infectious Disease journal PY - 2015 VL - 21 IS - 3 SP - 400 SN - 1080-6059 AB - Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics. KW - influenza KW - influenza A KW - nanomicroarray KW - next-generation sequencing KW - viruses KW - influenza virus KW - RNA KW - DNA KW - genes KW - hemagglutinin KW - neuraminidase KW - subtype DO - 10.3201/eid2103.141169 UR - https://wwwnc.cdc.gov/eid/article/21/3/14-1169_article ER - End of Reference