Figure 2. Molecular identification of parasites collected from 3 dogs in southern California, USA, by using sequence from the rRNA internal spacer (ITS). A) Multiple sequence alignment of ITS sequences from the 3 dogs and sequences from various Onchocerca parasites. Gray shading indicates areas of sequence identity. B) Unrooted phylogeny of the sequences shown in panel A. Numbers refer to the percentage of times the grouping distal to the number was supported in a bootstrap analysis of 1,000 replicate datasets. GenBank accession numbers for the sequences used in the alignment and phylogeny were as follows: O. lupi, JX489168; dog B, KC763782; dog C, KC763781; dog A, KC763779; O. ochengi, DQ523781; O. cervicalis, U13678; O. volvulus, AF325546. Scale bar indicates nucleotide substitutions per 100 residues.