TY - JOUR AU - Hassan, Hassan AU - Bolcen, Shanna AU - Kubofcik, Joseph AU - Nutman, Thomas AU - Eberhard, Mark AU - Middleton, Kelly AU - Wekesa, Joseph Wakoli AU - Ruedas, Gimena AU - Nelson, Kimberly AU - Dubielzig, Richard AU - De Lombaert, Melissa AU - Silverman, Bruce AU - Schorling, Jamie AU - Adler, Peter AU - Unnasch, Thomas AU - Beeler, Emily T1 - Isolation of Onchocerca lupi in Dogs and Black Flies, California, USA T2 - Emerging Infectious Disease journal PY - 2015 VL - 21 IS - 5 SP - 789 SN - 1080-6059 AB - In southern California, ocular infections caused by Onchocerca lupi were diagnosed in 3 dogs (1 in 2006, 2 in 2012). The infectious agent was confirmed through morphologic analysis of fixed parasites in tissues and by PCR and sequencing of amplicons derived from 2 mitochondrially encoded genes and 1 nuclear-encoded gene. A nested PCR based on the sequence of the cytochrome oxidase subunit 1 gene of the parasite was developed and used to screen Simulium black flies collected from southern California for O. lupi DNA. Six (2.8%; 95% CI 0.6%–5.0%) of 213 black flies contained O. lupi DNA. Partial mitochondrial16S rRNA gene sequences from the infected flies matched sequences derived from black fly larvae cytotaxonomically identified as Simulium tribulatum. These data implicate S. tribulatum flies as a putative vector for O. lupi in southern California. KW - parasites KW - California KW - Onchocerca lupi KW - dogs KW - black flies KW - zoonoses KW - filariasis KW - potcast KW - United States DO - 10.3201/eid2105.142011 UR - https://wwwnc.cdc.gov/eid/article/21/5/14-2011_article ER - End of Reference