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Volume 21, Number 8—August 2015
Dispatch

Detection and Full-Length Genome Characterization of Novel Canine Vesiviruses

Vito MartellaComments to Author , Pierfrancesco Pinto, Eleonora Lorusso, Barbara Di Martino, Qiuhong Wang, Vittorio Larocca, Alessandra Cavalli, Michele Camero, Nicola Decaro, Krisztián Bányai, Linda J. Saif, and Canio Buonavoglia
Author affiliations: Università Aldo Moro di Bari, Valenzano, Italy (V. Martella, P. Pinto, E. Lorusso, V. Larocca, A. Cavalli, M. Camero, N. Decaro, C. Buonavoglia); Università degli Studi di Teramo, Teramo, Italy (B. Di Martino); The Ohio State University, Wooster, Ohio, USA (Q. Wang, L.J. Saif); Hungarian Academy of Sciences, Budapest, Hungary (K. Bányai)

Main Article

Table

Primers used in study of the detection and full-length genome characterization of novel canine vesiviruses

Primer Position* Sequence Sense Use†
493F 5127–5150 GGT TTG CCA TCT GGC ATG CCG CTA + Detection
526R 5793–5816 AGC CAT VGC TCA RTT CTC AAA CAC − Detection
p289 5271–5292 TGA CAA TGT AAT CAT CAC CAT A − Detection
p290 4962–4984 GAT TAC TCC AAG TGG GAC TCC AC + Detection
501F 554–577 GTC TTG TGC TCT VTA CGA CAM ATG + Sequencing
VN3T20 3′- poly A GAG TGA CCG CGG CCG CT20. − 3′ RACE
502F 1215–1234 ATG ATW ATT GAH AAC CAY GA + Sequencing
509F 2904–2923 TAC GAT ATG GCY TGG GCY CT + Sequencing
510F 3411–3432 GAT GAT GAG TAC GAT GAR TGG A + Sequencing
511F 3588–3607 GAA GAC GTC ACC RTA ATT GG + Sequencing
513F 3876–3898 GTT ACG TTC RAT GGY GAA TTG GC + Sequencing
515F 4314–4338 CAC GTG TCA CCA GCA CAC RTD GAT G + Sequencing
504R 1599–1618 ACC ACG CTY TCR TTS GAC CA − Sequencing
528R 2887–2909 CTT GTC ATC TTA GTG TAC AAT GA − Sequencing
506R 1680–1702 AG GTT GGT RAC NGC RTC AAT GTC − Sequencing
508R 1974–1993 GT GTA GGC RYC GTG GTG GTC − Sequencing
512R 3588–3608 GCC AAT TAY GGT GAC RTC WTC − Sequencing
536F 714–732 TAC GAT CTT GCA ATC AAT G + Sequencing
GE72F 1915–1935 CCT ATG CCA TTG CGT CTA GAC + Sequencing
GE73R 2951–2970 CAG CCT TAA GTG CCT GCC AC − Sequencing
SEQ100 5634–5653 TGT CGC CAA ATG TTG ATG AG + Sequencing
SEQ101 6343–6360 TTG CCA CAG GCA CTC AGC + Sequencing
SEQ102 6860–6877 GGA AAC ACG TGG TGG TCA + Sequencing
SEQ103 7487–7504 AAG TAG AAT GAT TGG TGA + Sequencing
SEQ104 8063–8082 GAG TTT GAC AAG ATG AAC AG + Sequencing
GSP1 497–520 GCT TCA GAG ATC AGA ATA TCG TTG − 5′ RACE
GSP2 366–385 GTG GTC AGA GCC TTG GTC AG − 5′ RACE

*Position is based on the sequence of strain Bari/212/07/ITA (GenBank accession no. JN204722).
†RACE, rapid amplification of cDNA ends.

Main Article

Page created: July 15, 2015
Page updated: July 15, 2015
Page reviewed: July 15, 2015
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