Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 1—January 2016
Dispatch

Severe Community-Acquired Bloodstream Infection with Acinetobacter ursingii in Person who Injects Drugs

Tables
Article Metrics
6
citations of this article
EID Journal Metrics on Scopus
Helmut J.F. Salzer, Thierry Rolling, Stefan Schmiedel, Eva-Maria Klupp, Christoph Lange, and Harald SeifertComments to Author 
Author affiliations: University Medical Center Hamburg–Eppendorf, Hamburg, Germany (H.J.F. Salzer, T. Rolling, S. Schmiedel, E.-M. Klupp); Research Center Borstel, Sülfeld, Germany (H.J.F. Salzer, C. Lange); Karolinska Institute, Stockholm, Sweden (C. Lange); University of Namibia School of Medicine, Windhoek, Namibia (C. Lange); German Center for Infection Research, Braunschweig, Germany (C. Lange, H. Seifert); University of Cologne, Cologne, Germany (H. Seifert)

Cite This Article

Abstract

We report a community-acquired bloodstream infection with Acinteobacter ursingii in an HIV-negative woman who injected drugs. The infection was successfully treated with meropenem. Species identification was performed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Improved identification of Acinetobacter spp. by using this method will help identify clinical effects of this underdiagnosed pathogen.

Actinetobacter spp. are a diverse group of gram-negative, aerobic coccobacilli. Currently, >50 Acinetobacter spp. are recognized; most are not pathogenic to humans. Most human infections are nosocomial and predominantly caused by A. baumannii and other members of the A. baumannii group, including A. nosocomialis, A. pittii, and A. seifertii (1). Novel species, such as A. ursingii and A. schindleri, which have the potential to cause severe opportunistic infections, are emerging (28). Community-acquired Acinetobacter spp. infections are reported mainly from tropical regions. Most patients infected have community-acquired pneumonia caused by A. baumannii (1). We report a case of community-acquired bloodstream infection with A. ursingii in an HIV-negative woman who injected drugs.

The Case-Patient

In March 2014, a 47-year-old woman was hospitalized at the Bernhard Nocht Clinic (Hamburg, Germany) with chills, nausea, and fever (temperature <40°C). The patient had a history of intravenous drug use (cocaine and benzodiazepines) and was currently enrolled in a methadone substitution program. She also had chronic hepatitis C virus infection that was successfully treated with pegylated interferon-α, ribavirin, and boceprevir. The patient had a sustained virologic response and negative results for hepatitis C virus RNA at follow-up without progression to liver fibrosis or cirrhosis.

At admission, her peripheral blood leukocyte count was 6.6 × 103 cells/μL, and her C-reactive protein level was increased (29 mg/L [reference value <5 mg/L]). Other routine serum and urine chemical test results were unremarkable. HIV-1/2 infection was not detected. Chest radiograph showed no abnormalities. Soft tissue abscess was not observed, but the patient admitted having injected cocaine and unknown crushed tablets intravenously the day before admission. Toxicologic screening showed highly increased levels of benzodiazepine (>5,000 ng/mL) and cocaine (>1,000 ng/mL) (reference values <20 ng/mL for both substances). Three blood cultures were collected before empiric antimicrobial therapy with intravenous ceftriaxone (2 g 1×/d) was given on day 1.

The next day, the patient’s condition deteriorated. Her leukocyte count increased to 27.6 ×103 cells/μL, and C-reactive protein level increased to 112 mg/L. She was highly febrile and became hypotensive and tachycardic, which are compatible with severe sepsis. The patient responded rapidly to fluid substitution, became hemodynamically stable, and did not need vasopressors. Antimicrobial therapy was changed empirically to meropenem (1,000 mg 3×/d) on day 2.

After 18 h of culture incubation, microbial growth was detected in 3 aerobic blood culture bottles inoculated at admission. Gram staining showed gram-negative rods, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry fingerprinting with direct sample deposition without extraction (Bruker Daltonim GmbH, Bremen, Germany) identified A. ursingii (score 2.19). A BLAST search (http://www.ncbi.nlm.nih.gov/BLAST) of partial 16S rRNA gene sequence was performed by using a taxonomy browser (http://www.ncbi.nlm.nih.gov) and showed 99% identity with the A. ursingii type strain NBRC110605 (GenBank accession no. LC014147.1).

Antimicrobial susceptibility testing was performed by using Vitek 2 (bioMérieux, Nürtingen, Germany). Results showed susceptibility to imipenem (MIC <0.25 mg/mL), meropenem (<0.25 mg/mL), gentamicin (<1 mg/mL), and ciprofloxacin (<0.25 mg/mL) and resistance to ceftriaxone (32 mg/mL).

The patient became afebrile the next day, her clinical symptoms improved, and laboratory parameters of inflammation returned to reference values. Follow-up cultures remained negative. A transesophageal echocardiogram was unremarkable, without any evidence of infective endocarditis. Antimicrobial drug therapy was continued for 10 days before discharge. At follow-up 2 months later, the patient had no symptoms and was attending group-counseling sessions to maintain drug abstinence.

Conclusions

After the first description of A. ursingii as a new species (2), for which 13 of 29 A. ursingii isolates were obtained from patients with nosocomial bloodstream infections, few cases of A. ursingii bloodstream infections have been reported (Table). We were not able to identify cases of community-acquired A. ursingii bloodstream infections in the literature. Loubinoux et al. reported a case of A. ursingii bloodstream infection in a severely immunocompromised patient with pulmonary adenocarcinoma who received chemotherapy and corticosteroids (3). In that study, A. ursingii was associated with an implanted port device and considered of low pathogenic potential.

However, in the past few years, there have been reports of serious A. ursingii bloodstream infections in immunocompromised and immunocompetent patients. Horii et al. reported nosocomial A. ursingii bloodstream infections in 2 pregnant women who had no concurrent illnesses (4), but the route of transmission was not elucidated. The authors hypothesized that infections might have been acquired from intravenous catheters from the ward shower bath, where A. ursingii and 2 different strains of A. junii were found. A. ursingii was also found associated with nosocomial bloodstream infections among newborns, as demonstrated by 2 outbreaks in neonatal intensive care units that had high mortality rates. In both outbreaks, the source of infection could not be identified. In outbreaks reported by Kilic et al. (5) and Máder et al. (6), catheter-related infections were assumed. Kilic et al. (5) identified the organisms as A. septicus sp. nov., which were later shown by Nemec et al. (7) to be identical to A. ursingii. Dortet et al. (8) reported 10 A. ursingii infections, which included 3 cases of nosocomial bloodstream infections, but they did not provide clinical details.

Although severe infections with A. ursingii are rare, identification of A. ursingii by molecular methods, such as 16S rRNA gene sequencing, rpoB gene sequence cluster analysis, and amplified fragment length polymorphism fingerprinting, indicates a relatively high prevalence of A. ursingii (range 2.6%–4.5%) among clinical Acinetobacter spp. isolates (1013). In a study from the United Kingdom, A. ursingii was identified in 28 (4%) of 690 clinical Acinetobacter spp. isolates collected over a 20-month period during 2008–2009; a total of 17 (71%) of 24 A. ursingii isolates were recovered from blood cultures, but clinical details were not reported (12). Karah et al. (13) reported 113 case-patients with Acinetobacter spp. bloodstream infections in Norway; 3 (2.6%) were with A. ursingii, but clinical details of these patients were not provided. This relatively high incidence is consistent with results from the Netherlands (10) and Northern Ireland (11).

In most reports, A. ursingii isolates were more susceptible to antimicrobial drugs than A. baumannii isolates (4,6,8,12,13). However, Endo et al. (9) reported an IMP-1–producing, carbapenem-resistant A. ursingii isolate from a patient in Japan with a nosocomial bloodstream infection.

A. ursingii isolates are usually resistant to cephalosporins (3,5,8), as was the isolate from the patient we report, which explains the progression from severe sepsis when she was empirically given ceftriaxone to rapid clinical improvement after administration of meropenem. Máder et al. (6) reported a premature infant with an A. ursingii bloodstream infection also given a cephalosporin (ceftazidime). However, the infant received a combination with amikacin and immunoglobulins for 3 weeks. The infant survived and was discharged.

These findings suggest that A. ursingii is an emerging and serious clinical pathogen that is often involved in nosocomial bloodstream infections and associated with use of intravascular catheters. Its natural habitat remains unclear, but so far, it has only been isolated from human clinical sources. The frequency and clinical role of A. ursingii might have been underestimated because species identification by phenotypic and semiautomated identification methods is unreliable. For example, Vitek 2 has misidentified A. ursingii as Bordetella bronchiseptica (6,8). The most recent software version for Vitek 2 enabled unambiguous identification of A. ursingii (H. Seifert, pers. comm.).

We report a case of community-acquired bloodstream infection and severe sepsis caused by A. ursingii that was probably associated with intravenous drug use in an immunocompetent patient. This report also highlights the usefulness of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of Acinetobacter spp. We believe that this technology might improve correct species identification of Acinetobacter spp., including A. ursingii, in routine clinical practice and help elucidate the prevalence and clinical role of this emerging pathogen.

Dr. Salzer is a fellow at the Division of Clinical Infectious Diseases, Research Center Borstel, Süfeld, Germany. His primary research interests include invasive and chronic fungal infections.

Top

Acknowledgment

We thank the patient for providing permission to publish the case.

Top

References

  1. Peleg  AY, Seifert  H, Paterson  DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008;21:53882 . DOIPubMedGoogle Scholar
  2. Nemec  A, De Baere  T, Tjernberg  I, Vaneechoutte  M, van der Reijden  TJ, Dijkshoorn  L. Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov. isolated from human clinical specimens. Int J Syst Evol Microbiol. 2001;51:18919. DOIPubMedGoogle Scholar
  3. Loubinoux  J, Mihaila-Amrouche  L, Le Fleche  A, Pigne  E, Huchon  G, Grimont  PA, Bacteremia caused by Acinetobacter ursingii. J Clin Microbiol. 2003;41:13378. DOIPubMedGoogle Scholar
  4. Horii  T, Tamai  K, Mitsui  M, Notake  S, Yanagisawa  H. Bloodstream infections caused by Acinetobacter ursingii in an obstetrics ward. Infect Genet Evol. 2011;11:526. DOIPubMedGoogle Scholar
  5. Kilic  A, Li  H, Mellmann  A, Basustaoglu  AC, Kul  M, Senses  Z, Acinetobacter septicus sp. nov. association with a nosocomial outbreak of bacteremia in a neonatal intensive care unit. J Clin Microbiol. 2008;46:9028. DOIPubMedGoogle Scholar
  6. Máder  K, Terhes  G, Hajdu  E, Urban  E, Soki  J, Magyar  T, Outbreak of septicaemic cases caused by Acinetobacter ursingii in a neonatal intensive care unit. Int J Med Microbiol. 2010;300:33840. DOIPubMedGoogle Scholar
  7. Nemec  A, Musílek  M, Vaneechoute  M, Falsen  E, Dijkshoorn  L. Lack of evidence for “Acinetobacter septicus” as a species different from Acinetobacter ursingii? J Clin Microbiol. 2008;46:28267. DOIPubMedGoogle Scholar
  8. Dortet  L, Legrand  P, Soussy  CJ, Cattoir  V. Bacterial identification, clinical significance, and antimicrobial susceptibilities of Acinetobacter ursingii and Acinetobacter schindleri, two frequently misidentified opportunistic pathogens. J Clin Microbiol. 2006;44:44718. DOIPubMedGoogle Scholar
  9. Endo  S, Sasano  M, Yano  H, Inomata  S, Ishibashi  N, Aoyagi  T, IMP-1-producing carbapenem-resistant Acinetobacter ursingii from Japan. J Antimicrob Chemother. 2012;67:25334. DOIPubMedGoogle Scholar
  10. Boo  TW, Crowley  B. Detection of blaOXA-58 and blaOXA-23-like genes in carbapenem-susceptible Acinetobacter clinical isolates: should we be concerned? J Med Microbiol. 2009;58:83941. DOIPubMedGoogle Scholar
  11. van den Broek  PJ, van der Reijden  TJ, van Strijen  E, Helmig-Schurter  AV, Bernards  AT, Dijkshoorn  L. Endemic and epidemic Acinetobacter species in a university hospital, an eight years’ survey. J Clin Microbiol. 2009;47:35939. DOIPubMedGoogle Scholar
  12. Turton  JF, Shah  J, Ozongwu  C, Pike  R. Incidence of Acinetobacter species other than A. baumannii among clinical isolates of Acinetobacter: evidence for emerging species. J Clin Microbiol. 2010;48:14459. DOIPubMedGoogle Scholar
  13. Karah  N, Haldorsen  B, Hegstad  K, Simonsen  GS, Sundsfjord  A, Samuelsen  Ø. Norwegian Study Group of Acinetobacter. Species identification and molecular characterization of Acinetobacter spp. blood culture isolates from Norway. J Antimicrob Chemother. 2011;66:73844. DOIPubMedGoogle Scholar

Top

Table

Top

Cite This Article

DOI: 10.3201/eid2201.151298

Table of Contents – Volume 22, Number 1—January 2016

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Harald Seifert, Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstrasse 19-21, 50935 Cologne, Germany

Send To

10000 character(s) remaining.

Top

Page created: December 18, 2015
Page updated: December 18, 2015
Page reviewed: December 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external