Volume 22, Number 10—October 2016
Letter
Unmet Needs for a Rapid Diagnosis of Chikungunya Virus Infection
Elisa Burdino
, Guido Calleri, Pietro Caramello, and Valeria Ghisetti
, Guido Calleri, Pietro Caramello, and Valeria Ghisetti
Table
Reported sensitivity and specificity of rapid point-of-care tests for detecting chikungunya virus, 2008–2015*
| Reference and test(s) | Time from symptom onset to testing, d | Sensitivity, %‡ | Specificity, %‡ | Test reference standard |
|---|---|---|---|---|
| (1) | ||||
| OnSite Chikungunya IgM Rapid Test | 1 to >21 | 20.5 | 100 | Capture ELISA IgM (in house) with Asian lineage virus; rRT-PCR |
| SD BIOLINE Chikungunya IgM test |
1 to >21 |
50.8 |
89.2 |
Capture ELISA IgM (in house) with Asian lineage virus; rRT-PCR |
| (2) | ||||
| SD BIOLINE Chikungunya IgM test |
<7; 8 to >14§ |
22; 83 |
88; 71 |
ELISA IgM; rRT-PCR |
| (3) | ||||
| OnSite Chikungunya IgM Rapid Test |
3.75 to >7 |
12.1 |
100 |
IgM IFA; capture ELISA IgM (in house); rRT-PCR |
| (4) | ||||
| SD BIOLINE Chikungunya IgM test |
3–8 |
1.9–3.9 |
92.5–95.0 |
Capture ELISA IgM; rRT-PCR |
| (5) | ||||
| OnSite Chikungunya IgM Combo Rapid Test CE | NA | 20 | 93 | Capture ELISA IgM/IgG (in house); plaque reduction neutralization test |
| SD BIOLINE Chikungunya IgM test |
NA |
30 |
73 |
Capture ELISA IgM/IgG (in house); plaque reduction neutralization test |
| This study | ||||
| OnSite Chikungunya IgM Combo Rapid Test CE | 7 to 30 | 37.5 | 100 | IFA IgM/IgG (commercial); rRT-PCR |
*IFA, indirect immunofluorescence assay; NA, not applicable; rRT-PCR, real-time reverse transcription PCR.
†Manufacturers: CTK Biotech, San Diego, CA, USA (OnSite Chikungunya IgM Combo Rapid Test CE and OnSite Chikungunya IgM Rapid Test); Standard Diagnostics, Inc., Seoul, South Korea (SD BIOLINE Chikungunya IgM test).
‡Values are those reported in the original publications.
§Testing was done at 2 different time points after symptom onset.
References
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