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Volume 22, Number 12—December 2016

Zika Virus Infection in the Central Nervous System and Female Genital Tract

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To the Editor: On April 9, 2016, a 32-year-old woman from Italy traveled to Santo Domingo in the Dominican Republic. She worked as a volunteer nurse in the outpatient clinic of a primary school of a nongovernmental organization based in Italy. She returned to Italy on April 17. She did not have sexual intercourse during her stay abroad.

On April 26, she was referred to the travel clinic of the National Institute for Infectious Diseases Lazzaro Spallanzani in Rome for a febrile syndrome with rash, generalized headache, and weakness, which started on April 21. Approximately 24 hours later, she was admitted to the institute’s medical facility for a suspected neurologic involvement. At admission, she had abnormal gait, strong asthenia, and a disseminated pruritic rash on her face, abdomen, chest, and arms, but she did not have a fever.

During physical examination, the patient was alert and fully oriented. Temperature was 36.9°C, pulse rate 90 beats/min, blood pressure 100/60 mm Hg, and respiratory rate 20 breaths/min. She had a diffuse erythematous macular rash and bilateral nonpurulent conjunctival hyperemia without meningeal signs. Findings of a neurologic examination of the upper limbs were within reference ranges.. Muscular strength was reduced in both legs (left > right), whereas tendon reflexes and all sensory modalities were within reference ranges. Results of a contrast-enhanced magnetic resonance imaging of the brain and spinal cord (on day 7), nerve conduction studies and electromyography (on day 8), and an electroencephalogram (on day 16) were within reference ranges. A lumbar puncture (on day 7) showed normal cell counts (<10 cells/mL), a normal glycorrachia/glycemia ratio (>0.5), and a slight increase in protein concentration (0.48 g/L [reference range 0.32−0.80 g/L]) in cerebrospinal fluid. Complete neuropsychologic examinations (on days 9 and 10) showed mild deficits in attention and mental processing speed and mental flexibility and moderate deficits in verbal and nonverbal memory tasks (Technical Appendix).

Real-time reverse transcription PCR (rRT-PCR) results for dengue viruses 1–4 and chikungunya virus were negative in serum and cerebrospinal fluid (CSF), whereas Zika virus RNA was detected in serum (day 7), urine (up to day 27), CSF (day 6), saliva (up to day 13), and vaginal swab (up to day 13) (Technical Appendix). Specific dengue and chikungunya IgG and IgM were not detected in serum and CSF. Zika virus IgM was detected in serum starting on day 6. Zika virus–specific antibodies in serum were confirmed by microneutralization assay (Table).

Starting on day 7, intravenous polyvalent immunoglobulins were administered (0.4 g/kg/day for 5 days); no adverse events were observed. A second neuropsychologic examination was performed on day 16 and indicated persistent impairment in memory performances and an improvement in mental concentration and flexibility tasks (Technical Appendix).

A second lumbar puncture (on day 17) showed an increased cell count (70 cells/mL, mostly lymphocytes), and CSF was negative for Zika virus RNA by rRT-PCR. The patient was discharged on day 20; she showed a progressive neurologic recovery starting on day 16. At 60-days follow-up visit, no neurologic deficits were reported.

During the 2013–2014 outbreak of Zika virus in French Polynesia and in the context of the 2015–2016 Zika virus circulation (1), an apparent increase in Guillain-Barré syndrome incidence was reported. Few anecdotal cases of encephalopathy in patients with Zika virus infection have been recently described in affected countries: 1 case in a man on a 4-week cruise through an area in the South Pacific that included New Caledonia, Vanuatu, the Solomon Islands, and New Zealand in 2015 (2); and 2 cases in Martinique (3) in February 2016. Recently, Zika virus has been detected in the genital tract of a virus-infected woman after Zika virus had disappeared from blood and urine (4), and a suspected case of Zika virus by sexual transmission from a woman to a man has been reported in New York City (5).

In our patient, Zika virus RNA was found in different systems, including the central nervous system and the genital tract. Recently, a mouse model of Zika virus infection by vaginal exposure demonstrated that Zika virus replicated within the genital mucosa, persisted postinfection, and was detected in the fetal brain of the mice (6). In our case, the patient reported early neurologic symptoms and moderate memory impairment in neuropsychologic examinations, all features consistent with the diagnosis of Zika virus–related encephalitis, which represents a rare atypical presentation, particularly in areas to which Zika virus infection is not endemic. A recent article shows that Zika virus can infect adult murine neural stem cells, leading to cell death and reduced proliferation (7). It raises the possibility that Zika is not simply a transient infection in adult humans and that exposure in the adult brain could have an effect on long-term memory or the risk for depression (7).

Our case highlights the potential for Zika virus neurotropism and the need for early identification of Zika virus–related neurologic symptoms. Moreover, the presence of Zika virus in the genital tract supports the recommendation of safe sex practice for women returning home from areas with ongoing Zika virus transmission.



The study was funded by Ricerca Corrente of the Italian Ministry of Health.


Emanuele Nicastri, Concetta CastillettiComments to Author , Pietro Balestra, Simonetta Galgani, and Giuseppe Ippolito
Author affiliations: National Institute for Infectious Diseases Lazzaro Spallanzani, Rome, Italy (E. Nicastri, C. Castilletti, P. Balestra, G. Ippolito); Azienda Ospedaliera San Camillo-Forlanini, Rome (S. Galgani)



  1. European Centre for Disease Prevention and Control. Rapid risk assessment. Zika virus disease epidemic. Sixth update, 20 May 2016. Stockholm: The Centre; 2016 [cited 2016 Aug 1].
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  3. Rozé  B, Najioullah  F, Signate  A, Apetse  K, Brouste  Y, Gourgoudou  S, et al.; Neuro-Zika Working Group of Martinique. Zika virus detection in cerebrospinal fluid from two patients with encephalopathy, Martinique, February 2016. Euro Surveill. 2016;21:30205.DOIPubMedGoogle Scholar
  4. Prisant  N, Bujan  L, Benichou  H, Hayot  P-H, Pavili  L, Lurel  S, et al. Zika virus in the female genital tract. Lancet Infect Dis. 2016;pii: S1473-3099(16)30193-1. DOIGoogle Scholar
  5. Davidson  A, Slavinski  S, Komoto  K, Rakeman  J, Weiss  D. Suspected female-to-male sexual transmission of Zika virus—New York City, 2016. MMWR Morb Mortal Wkly Rep. 2016;65):716–7. DOIGoogle Scholar
  6. Yockey  LJ, Varela  L, Rakib  T, Khoury-Hanold  W, Fink  SL, Stutz  B, et al. Vaginal exposure to Zika virus during pregnancy leads to fetal brain infection. Cell. 2016;166:12471256.e4.DOIPubMedGoogle Scholar
  7. Li  H, Saucedo-Cuevas  L, Regla-Nava  JA, Chai  G, Sheets  N, Tang  W, et al. Zika virus infects neural progenitors in the adult mouse brain and alters proliferation. Cell Stem Cell. 2016;pii:1934-5909(16)30252-1. DOIGoogle Scholar




Cite This Article

DOI: 10.3201/eid2212.161280

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Table of Contents – Volume 22, Number 12—December 2016

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Concetta Castilletti, National Institute for Infectious Diseases INMI-IRCCS, Via Portuense 292, 00149 Rome, Italy

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Page created: November 18, 2016
Page updated: November 18, 2016
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