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Volume 22, Number 2—February 2016
Dispatch

Bordetella pertussis Strain Lacking Pertactin and Pertussis Toxin

Margaret M. WilliamsComments to Author , Kathryn Sen, Michael R. Weigand, Tami H. Skoff, Victoria A. Cunningham, Tanya A. Halse, M. Lucia Tondella, and CDC Pertussis Working Group
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.M. Williams, M.R. Weigand, T.H. Skoff, M.L. Tondella); New York State Department of Health, Albany, New York, USA (K.A. Sen); Livingston County Health Department, Mt. Morris, New York, USA (V.A. Cunningham); Wadsworth Center, Albany (T.A. Halse)

Main Article

Table 1

Characterization of Bordetella pertussis strains I979 and FR3749 in comparison to strain Tohama I*

Strain GenBank accession no. Length, bp No. IS481 prn type Prn Reference
FR3749 CP010965 4,079,396 249 2 + This study
I979 CP010966 4,082,551 252 2 – This study
Tohama I
 NC_002929.2
 4,086,189
 238
 1
 +
 (10)
*Prn, pertactin, +, positive; –, negative.

Main Article

References
  1. Misegades  LK, Winter  K, Harriman  K, Talarico  J, Messonnier  NE, Clark  TA, Association of childhood pertussis with receipt of 5 doses of pertussis vaccine by time since last vaccine dose, California, 2010. JAMA. 2012;308:2126–32. DOIPubMedGoogle Scholar
  2. Pawloski  LC, Queenan  AM, Cassiday  PK, Lynch  AS, Harrison  MJ, Shang  W, Prevalence and molecular characterization of pertactin-deficient Bordetella pertussis in the United States. Clin Vaccine Immunol. 2014;21:119–25 . DOIPubMedGoogle Scholar
  3. Bowden  KE, Williams  MM, Cassiday  PK, Milton  A, Pawloski  L, Harrison  M, Molecular epidemiology of the pertussis epidemic in Washington State in 2012. J Clin Microbiol. 2014;52:3549–57. DOIPubMedGoogle Scholar
  4. Bouchez  V, Brun  D, Cantinelli  T, Dore  G, Njamkepo  E, Guiso  N. First report and detailed characterization of B. pertussis isolates not expressing pertussis toxin or pertactin. Vaccine. 2009;27:6034–41. DOIPubMedGoogle Scholar
  5. Probert  WS, Ely  J, Schrader  K, Atwell  J, Nossoff  A, Kwan  S. Identification and evaluation of new target sequences for specific detection of Bordetella pertussis by real-time PCR. J Clin Microbiol. 2008;46:3228–31. DOIPubMedGoogle Scholar
  6. Skoff  TH, Baumbach  J, Cieslak  PR. Tracking pertussis and evaluating control measures through enhanced pertussis surveillance, Emerging Infections Program, United States. Emerg Infect Dis. 2015;21:1568. DOIPubMedGoogle Scholar
  7. Tatti  KM, Sparks  KN, Boney  KO, Tondella  ML. Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens. J Clin Microbiol. 2011;49:4059–66. DOIPubMedGoogle Scholar
  8. Schmidtke  AJ, Boney  KO, Martin  SW, Skoff  TH, Tondella  ML, Tatti  KM. Population diversity among Bordetella pertussis isolates, United States, 1935–2009. Emerg Infect Dis. 2012;18:1248–55. DOIPubMedGoogle Scholar
  9. Hardwick  TH, Cassiday  P, Weyant  RS, Bisgard  KM, Sanden  GN. Changes in predominance and diversity of genomic subtypes of Bordetella pertussis isolated in the United States, 1935 to 1999. Emerg Infect Dis. 2002;8:44–9 and. DOIPubMedGoogle Scholar
  10. Parkhill  J, Sebaihia  M, Preston  A, Murphy  LD, Thomson  N, Harris  DE, Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat Genet. 2003;35:32–40. PubMed DOIGoogle Scholar
  11. Martin  SW, Pawloski  L, Williams  M, Weening  K, DeBolt  C, Qin  X, Pertactin-negative Bordetella pertussis strains: evidence for a possible selective advantage. Clin Infect Dis. 2015;60:223–7. DOIPubMedGoogle Scholar
  12. Imaizumi  A, Suzuki  Y, Ono  S, Sato  H, Sato  Y. Effect of heptakis(2,6-O-dimethyl)β-cyclodextrin on the production of pertussis toxin by Bordetella pertussis. Infect Immun. 1983;41:1138–43 .PubMedGoogle Scholar
  13. Bart  MJ, Harris  SR, Advani  A, Arakawa  Y, Bottero  D, Bouchez  V, Global population structure and evolution of Bordetella pertussis and their relationship with vaccination. MBio. 2014;5:e01074. DOIPubMedGoogle Scholar
  14. Darling  AE, Mau  B, Perna  NT. progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS ONE. 2010;5:e11147. DOIPubMedGoogle Scholar
  15. King  AJ, van Gorkom  T, Pennings  JL, van der Heide  HG, He  Q, Diavatopoulos  D, Comparative genomic profiling of Dutch clinical Bordetella pertussis isolates using DNA microarrays: identification of genes absent from epidemic strains. BMC Genomics. 2008;9:311. DOIPubMedGoogle Scholar

Main Article

1Members of the CDC Pertussis Working Group are listed at the end of this article.

Page created: January 15, 2016
Page updated: January 15, 2016
Page reviewed: January 15, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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