TY - JOUR AU - Jensen, Anne Kvistholm AU - Björkman, Jonas AU - Ethelberg, Steen AU - Kiil, Kristoffer AU - Kemp, Michael AU - Nielsen, Eva Møller T1 - Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002–2012 T2 - Emerging Infectious Disease journal PY - 2016 VL - 22 IS - 4 SP - 625 SN - 1080-6059 AB - Denmark has a high incidence of invasive listeriosis (0.9 cases/100,000 population in 2012). We analyzed patient data, clinical outcome, and trends in pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) of Listeria monocytogenes strains isolated in Denmark during 2002–2012. We performed 2-enzyme PFGE and serotyping on 559 isolates and MLST on 92 isolates and identified some correlation between molecular type and clinical outcome and patient characteristics. We found 178 different PFGE types, but isolates from 122 cases belonged to just 2 closely related PFGE types, clonal complex 8 and sequence type 8. These 2 types were the main cause of a peak in incidence of invasive listeriosis during 2005–2009, possibly representing an outbreak or the presence of a highly prevalent clone. However, current typing methods could not fully confirm these possibilities, highlighting the need for more refined discriminatory typing methods to identify outbreaks within frequently occurring L. monocytogenes PFGE types. KW - listeriosis KW - Listeria monocytogenes KW - epidemiology KW - molecular epidemiology KW - pulsed-field gel electrophoresis KW - multilocus sequence typing KW - foodborne diseases KW - bacteria KW - humans KW - Denmark KW - zoonoses KW - Food Safety DO - 10.3201/eid2204.150998 UR - https://wwwnc.cdc.gov/eid/article/22/4/15-0998_article ER - End of Reference