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Volume 22, Number 7—July 2016

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Jesse J. Waggoner, Lionel Gresh, Alisha Mohamed-Hadley, Gabriela Ballesteros, Maria Jose Vargas Davila, Yolanda Tellez, Malaya K. Sahoo, Angel Balmaseda, Eva Harris, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (J.J. Waggoner, A. Mohamed-Hadley, M.K. Sahoo, B.A. Pinsky); Sustainable Sciences Institute, Managua, Nicaragua (L. Gresh); Laboratory Centro Nacional de Diagnóstico y Referencia of the Ministry of Health, Managua (G. Ballesteros, M.J. Vargas Davila, Y. Tellez, A. Balmaseda); School of Public Health of the University of California, Berkeley, Berkeley, California, USA (E. Harris)

Main Article

Table 2

Comparison of Zika virus detection in the ZCD assay and the Zika virus comparator rRT-PCR*

ZCD assay Zika virus rRT-PCR
Pos Neg Total
Pos 25 31† 56
Neg 1‡ 76 77
Total 26 107 133

*CHIKV, chikungunya virus; Ct, cycle threshold; DENV, dengue virus; neg, negative; pos, positive; rRT-PCR, real-time reverse transcription PCR; ZCD assay, single-reaction multiplex rRT-PCR for Zika virus, CHIKV, and DENV.
†22 samples produced a late Ct in the comparator Zika virus rRT-PCR (Ct >38.5)
‡Sample was also pos for a DENV-CHIKV co-infection and tested pos for Zika virus when repeated in the ZCD assay.

Main Article

Page created: June 14, 2016
Page updated: June 14, 2016
Page reviewed: June 14, 2016
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