Cutaneous Melioidosis Cluster Caused by Contaminated Wound Irrigation Fluid
Adam J. Merritt, Mariani Peck, Dionne Gayle, Avram Levy, Yi-Horng Ler, Edward Raby, Tristan M. Gibbs, and Timothy J.J. Inglis
Author affiliations: PathWest Laboratory Medicine, Nedlands, Western Australia, Australia (A.J. Merritt, M. Peck, D. Gayle, A. Levy, Y.-H. Ler, T.M. Gibbs, T.J.J. Inglis); University of Western Australia, Crawley, Western Australia, Australia (A.J. Merritt, T.J.J. Inglis); Royal Perth Hospital, Perth, Western Australia, Australia (E. Raby)
Figure 2. Bacterial culture results for 1,000-mL bottle of wound irrigation fluid in laboratory investigation of a 2012–2013 cutaneous melioidosis cluster in the temperate southern region of Western Australia. A) Wound irrigation fluid in original bottle. B) Direct primary culture of wound irrigation fluid on blood agar plate, showing growth inhibition of Pseudomonas aeruginosa and revealing Burkholderia pseudomallei around gentamicin disk. C) Filtrate of wound irrigation fluid from same bottle showing higher count of B. pseudomallei colonies than P. aeruginosa. D) Dilution of wound irrigation fluid (1:100), dispensed by spiral plating device, showing B. pseudomallei colonies and relatively sparse P. aeruginosa colonies.
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