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Volume 22, Number 8—August 2016

Phylogeographic Evidence for 2 Genetically Distinct Zoonotic Plasmodium knowlesi Parasites, Malaysia

Ruhani Yusof1, Md Atique Ahmed1, Jenarun Jelip, Hie Ung Ngian, Sahlawati Mustakim, Hani Mat Hussin, Mun Yik Fong, Rohela Mahmud, Frankie Anak Thomas Sitam, J. Rovie-Ryan Japning, Georges Snounou, Ananias A. Escalante, and Yee Ling LauComments to Author 
Author affiliations: University of Malaya Faculty of Medicine, Kuala Lumpur, Malaysia (R. Yusof, M.A. Ahmed, M.Y. Fong, R. Mahmud, Y.L. Lau); Sabah State Health Department, Sabah, Malaysia (J. Jelip); Hospital Kapit, Sarawak, Malaysia (H.U. Ngian); Hospital Tengku Microbiology Laboratory, Ampuan Rahimah, Selangor, Malaysia (S. Mustakim); Kelantan State Health Department, Kelantan, Malaysia (H.M. Hussin); Department of Wildlife and National Parks Peninsular Malaysia, Kuala Lumpur, Malaysia (F.A.T. Sitam, J. Rovie-Ryan); Sorbonne Universités, INSERM, Paris, France (G. Snounou); Temple University, Philadelphia, PA, USA (G. Snounou, A.A. Escalante)

Main Article

Table 2

Samples and sequences obtained for the PkCOX1 gene used to distinguish the 2 distinct Plasmodium knowlesi parasite populations in Malaysia*

Location No. sequences
Total sequences obtained
No. samples†
Human Macaque This study‡ GenBank§ This study‡ GenBank§
Peninsular Malaysia
Kelantan 13 ND 15 ND ND ND 15
Johor 3 ND 4 ND ND ND 4
Selangor 7 5 5 ND 5 1 11
Terengganu 2 ND 1 ND 0 ND 1
Pahang 14 1 18 ND 2 1 21
Negeri Sembilan


Malaysian Borneo
Sarawak 22 ND 23 21 ND 22 66


Total samples NA 86 78 22 8 12 100 human + 20 macaque
Total sequences NA NA 84 22 8 24 106 human + 32 macaque

*NA, not applicable; ND, no data; PkCOX1 P. knowlesi cytochrome oxidase subunit I.
†Previously collected samples used for this study.
‡GenBank accession nos.KT900705–KT900797).
§GenBank accession numbers listed in online Technical Appendix Table (

Main Article

1These authors contributed equally to this article.

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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