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Volume 22, Number 8—August 2016
Synopsis

Co-infections in Visceral Pentastomiasis, Democratic Republic of the Congo

Dennis TappeComments to Author , Mihály Sulyok, Therese Riu, Lajos Rózsa, Imre Bodó, Christoph Schoen, Birgit Muntau, Gergely Babocsay, and Richard Hardi
Author affiliations: Bernhard Nocht Institute, Hamburg, Germany (D. Tappe, B. Muntau); Eberhard Karls University Tübingen, Germany (M. Sulyok); Hôpital Géneral de Reference de Kole, Kole, Democratic Republic of the Congo (T. Riu); MTA-ELTE-MTM Ecology Research Group, Budapest, Hungary (L. Rózsa); Emory University School of Medicine, Atlanta, Georgia, USA (I. Bodó); University of Würzburg, Würzburg, Germany (C. Schoen); Mátra Museum of the Hungarian Natural History Museum, Gyöngyös, Hungary (G. Babocsay); St. Raphael Ophthalmological Center, Mbuji Mayi, Democratic Republic of the Congo (R. Hardi)

Main Article

Table

Patient characteristics and sequencing results of pentastomid 18S rDNA and Armillifer-specific cytochrome oxidase gene PCRs, Sankuru District, Democratic Republic of the Congo, 2014–2015*

Patient no. Age, y/sex Type of surgery Location of pentastomid cyst Sequencing result
 Histology
Pentastomid 18S rDNA-gene, 430 bp Armillifer cytochrome oxidase subunit I-gene, 288 bp
1†
 NA/M
 Laparotomy
 Peritoneum
 Cyst 1: A. grandis A. grandis Not done
Cyst 2: negative A. armillatus Not done
Cyst 3: Raillietiella sp.‡ Negative‡ Necrotic pentastomid
Cyst 4: negative§
 Negative§
 Necrotic pentastomid
2†
 35/F
 Laparotomy
 Peritoneum
 Cyst 1: A. grandis A. grandis Not done
Cyst 2: A. armillatus A. armillatus Not done
Cyst 3: negative‡ A. grandis‡ Necrotic pentastomid
Cyst 4: negative‡ A. grandis‡ Total necrosis
Cyst 5: negative‡ A. grandis‡ Necrotic pentastomid
Cyst 6: negative‡
 A. grandis‡
 Total necrosis
3†
 NA/M
 Laparotomy
 Peritoneum
 Cyst 1: A. grandis A. grandis Not done
Cyst 2: A. armillatus A. armillatus Not done.
Cyst 3: A. grandis Negative Not done
Cyst 4: negative§ A. grandis§ Total necrosis
Cyst 5: negative§ A. armillatus§ Total necrosis
Cyst 6: negative‡
 A. armillatus§
 Total necrosis
4
 NA/M
 Hernioplasty
 Peritoneum
 Cyst 1: A. armillatus
 A. armillatus
 Not done
5
 NA/F
 Cesarean section
 Omentum
 Cyst 1: A. armillatus A. armillatus Not done
Cyst 2: A. armillatus‡
 A. armillatus‡
 Necrotic pentastomid
6
 36/F
 Laparotomy, appendectomy
 Left ovary
 Cyst 1: A. armillatus A. armillatus Not done
Cyst 2: not done Not done Ovarian cyst
Cyst 3: not done
 Not done
 Ovarian cyst
7 42/F Laparotomy, appendectomy Omentum Cyst 1: A. armillatus A. armillatus Not done

*NA, not available; negative, PCR negative or no positive sequence result.
†Co-infected.
‡PCRs performed from paraffin block tissue slices.
§Repeat PCRs performed from whole paraffin block, after PCRs from block slices were negative. Tissue materials from patient 1 (cysts 1, 2), patient 3 (cyst 3), and patient 7 (cyst 1) were ground, as the tissue was too hard to cut into pieces for PCR.

Main Article

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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