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Volume 22, Number 9—September 2016
Research

Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections

Valérie Bordeau, Anne Cady, Matthieu Revest, Octavie Rostan, Mohamed Sassi, Pierre Tattevin, Pierre-Yves Donnio, and Brice FeldenComments to Author 
Author affiliations: University of Rennes 1, Rennes, France (V. Bordeau, A. Cady, M. Revest, O. Rostan, M. Sassi, P. Tattevin, P.-Y. Donnio, B. Felden); Institut National de la Santé et de la Recherche Médicale Unité 835, Rennes (V. Bordeau, M. Sassi, P. Tattevin, B. Felden); Rennes University Hospital, Rennes (A. Cady, M. Revest, P. Tattevin, P.-Y. Donnio)

Main Article

Table 2

Characteristics of 83 clinical strains of Staphylococcus aureus, Rennes, France*

Strain ID Strain clinical status MLST sequence type spa type Small pathogenicity island RNAs
 Core genome RNAs
sprA sprB sprC sprD sprX tmRNA 6S RNA RNAIII
Group 1
L253 Carriage 10 ZZ2PNGKBKGOLB + – + – – + + +
L341 Carriage 398 XKAOAOBO – – + + – + + +
7 Carriage 398 XKAOAOBO – – + + – + + +
9† Carriage 398 XKAOAOBO – – + + – + + +
16 Carriage 398 XKAOAOBO – – + + – + + +
18 Carriage 398 XKAOAOBO – – + + – + + +
20† Carriage 398 XKAOAOBO – – + + – + + +
430‡ Septic shock 398 XKAOAOBO – – + + – + + +
27‡ Septic shock 30 WGKAKAOAOMQ + – – + + + + +
23‡ Carriage 30 WGKAKAOMQQ + – – + + + + +
13 Carriage 30 WGKAKAOMQQ + – – + + + + +
22 Carriage 30 WGKAKAOMQ + – – + + + + +
28 Carriage 30 XKAKAOMQQ + – – + + + + +
52†‡ Sepsis 30 WGKAKAQ + – – + + + + +
46†‡ Sepsis 30 WGKAKBMQ + – – + + + + +
82†‡ Sepsis 30 WXKAKAOMQQ + – – + + + + +
2167†‡ Carriage 30 WGKBKAOMQQ + – – + + + + +
1954†‡ Carriage 30 WGKAKAOMQQQQ + – – + + + + +
KS‡ Septic shock 30 WGKAKAOMQQ + – – + + + + +
886‡ Septic shock 30 WGKAKAOMQQ + – – + + + + +
86‡ Sepsis 34 ZZ2PNGKBKGOLB + – – + + + + +
89‡ Septic shock 34 ZZ2PNGKBKGOLB + – – + + + + +
59‡ Sepsis 34 ZZ2PNGKBKGOMLLB + – – + + + + +
45‡ Septic shock 45 XKAKBEMB + – – – – + + +
34‡ Septic shock 45 XKAKBEMBKB – – – + + + + +
8‡ Carriage 45 XKAKEEMBKB – – – + + + + +
49‡ Sepsis 45 XKAKBEMBKB – – – + + + + +
2203†‡ Carriage 45 XKAKAKBEMBBKB – – – + + + + +
1911†‡ Carriage 45 A2AKBEEMBKBB – – – + + + + +
30‡ Septic shock 22 TJEJNF2MOMOK + – – + + + + +
L317 Carriage 22 TJEJNCMOMOKR + – – + + + + +
1955†‡§ Carriage 22 TJJEJNF2MNF2MOMOKR + – – + + + + +
2752‡ Carriage 22 TJEJCMOMOKR + – – + + + + +
6 Carriage 21 I2Z2EGMJH2M – + – + + + + +
14ঠSeptic shock 121 I2Z2EGMMJH2M + + Р+ + + + +
Group 2
18†‡ Sepsis 1 UJFKBPE + + + + + + + +
5 Carriage 5 TJMBMDMGMK + + + + + + + +
21‡ Carriage 5 TJMEMDMGGK + + + + + + + +
62द Sepsis 5 TMDGGK + + + + + (2) + + +
21 Carriage 5 TJMBMK + + + + + + + +
1899† Carriage 5 TJMBMK + + + + + + + +
1414† Carriage 5 TMDMGGMK + + + + + + + +
15†‡¶ Carriage 5 TJMBMDMGMK + + + + + + + +
88†‡¶ Sepsis 5 TJMBMDMGMK + + + + + + + +
104‡ Sepsis 7 UJFMBGJAGJ + – + + + + + +
17†‡¶ Septic shock 8 YHGFMBQBLO + + + – – + + +
26†‡§¶ Septic shock 8 YHGFMBQBLO + + + + + + + +
28ঠSeptic shock 8 YHGFMBQBLO + + + + + + + +
10†‡¶ Carriage 8 YHGFMBQBLO + + + + + + + +
L102†‡¶ Carriage 8 YHGFMBQBLO + + + + + + + +
L385 Carriage 8 YHBQBLO + + + + + + + +
L400 Carriage 8 YHFMBQBLO + + + + + + + +
1†‡§¶ Carriage 8 YHFMBQBLO + + + + + + + +
2§ Carriage 8 YHFMBQBLO + + + + + + + +
3§ Carriage 8 YHFMBQBLO + + + + + + + +
11‡ Carriage 8 YHGFMBQBLO + + + + – + + +
9†‡§¶ Sepsis 8 YHGFMBQBLO + + + + + + + +
20ठSepsis 8 YC2FMBQBLO + + + + + + + +
43†‡§¶ Sepsis 8 YHGFMBQBLO + + + + + (2) + + +
75†‡¶ Sepsis 8 YMBQBLO + + + + + + + +
100§ Sepsis 8 YHGFHGFMBQBLO + + + + + + + +
2155†‡ Carriage 8 YMBQLO + + + + + + + +
310†‡ Carriage 8 YHMBQBQBLO + + + + + + + +
54ठSepsis 8 YHGFMBQBLO + + + + + + + +
58‡ Sepsis 8 YHGFMBQBLO + + + + + + + +
85‡ Sepsis 8 YHGFMBQBLO + + + + + + + +
101ठSepsis 8 YHGFMBQBLO + + + + + + + +
11‡ Septic shock 15 UJGBBGGJAGJ – + + + + + + +
42‡ Septic shock 15 UJGBBGGJAG – + + + + + + +
106 Sepsis 15 UJGBBGGJ + + + + – + + +
4 Carriage 15 UJGBGGJAGJ + + + + + + + +
L45‡ Carriage 15 TJGBGGJAGJ – + + + + + + +
19†‡¶ Carriage 25 ZFGU2DMGM + + + + + + + +
91†‡¶ Sepsis 25 ZFGDMGM + + + + + + + +
61‡ Sepsis 25 ZFGU2DMGGMM + + + + + + + +
74‡ Sepsis 25 ZGU2DMGGM + + + + + + + +
71†‡
 Sepsis
 9
 I2GJAABB
 +
 –
 +
 +
 +
 +
 +
 +
44†‡¶ Septic shock 101 ZDGMDMGMM + – + + + (2) + + +
57‡ Septic shock 188 UJGFMB + + + + + + + +
26‡ Carriage 188 UJGFMB + + + + + + + +
12 Carriage 883 JJMMJJJJMK + – + – – + + +
69‡ Septic shock ND GFMGGM + + + – – + + +
65†§¶ Sepsis ND ND + + + + + + + +

*Values in parentheses indicate number of detected gene copies. ID, identification; MLST, multilocus sequence typing; spa, S. aureus protein A; spr, small pathogenicity island RNA; tm, transfer–messenger; +, positive; –, negative; ND, not determined. †Strains for which SaeR expression was analyzed by Western blot.
‡Strains for which quantitative PCR results and second immunoglobulin-binding protein expression was analyzed by Western blot.
§Strains resistant to methicillin.
¶Strains for which small RNA expression was analyzed by Northern blot.

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Page created: August 23, 2016
Page updated: August 23, 2016
Page reviewed: August 23, 2016
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