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Volume 22, Number 9—September 2016
Dispatch

Borrelia miyamotoi–Associated Neuroborreliosis in Immunocompromised Person

Katharina BodenComments to Author , Sabine Lobenstein, Beate Hermann, Gabriele Margos, and Volker Fingerle
Author affiliations: University Hospital Jena, Jena, Germany (K. Boden, B. Hermann); Burgenlandkreis Hospital Naumburg, Naumburg, Germany (S. Lobenstein); Bavarian Health and Food Safety Authority, Oberschleissheim, Germany (G. Margos, V. Fingerie)

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Figure 1

Molecular phylogenetic analysis of Borrelia strain from cerebrospinal fluid of a 74-year-old immunocompromised woman in Germany (black dot) conducted by using 6 multilocus sequence typing genes (clpA, clpX, pepX, pyrG, recG, rplB) of Borrelia miyamotoi. The sequences obtained from the patient sample clustered with B. miyamotoi strain EU_T01 from Europe, retrieved from the PubMLST Borrelia database (http://pubmlst.org/borrelia/). The phylogenetic relationship of the sample analyzed was inferred b

Figure 1. Molecular phylogenetic analysis of Borrelia strain from cerebrospinal fluid of a 74-year-old immunocompromised woman in Germany (black dot) conducted by using 6 multilocus sequence typing genes (clpA, clpX, pepX, pyrG, recG, rplB) of Borrelia miyamotoi. The sequences obtained from the patient sample clustered with B. miyamotoi strain EU_T01 from Europe, retrieved from the PubMLST Borrelia database (http://pubmlst.org/borrelia/). The phylogenetic relationship of the sample analyzed was inferred by using DNA sequences of chromosomal housekeeping genes. The maximum-likelihood method based on the general time reversible model (8) was applied. The tree with the highest log likelihood (–5531.9051) is shown. The percentage of trees in which the associated taxa clustered is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying neighbor-joining and BioNJ algorithms (http://bionj.org/about-bionj) to a matrix of pairwise distances estimated by using the maximum composite likelihood approach and then selecting the topology with superior log likelihood value. The tree is drawn to scale; branch lengths are measured in the number of substitutions per site. The analysis involved 5-nt sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. The final dataset contained 3,642 positions. Evolutionary analyses were conducted in MEGA5 (9).

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References
  1. Platonov  AE, Karan  LS, Kolyasnikova  NM, Makhneva  NA, Toporkova  MG, Maleev  VV, Humans infected with relapsing fever spirochete Borrelia miyamotoi, Russia. Emerg Infect Dis. 2011;17:1816–23. DOIPubMedGoogle Scholar
  2. Krause  PJ, Fish  D, Narasimhan  S, Barbour  AG. Borrelia miyamotoi infection in nature and in humans. Clin Microbiol Infect. 2015;21:631–9. DOIPubMedGoogle Scholar
  3. Telford  SR III, Goethert  HK, Molloy  PJ, Berardi  VP, Chowdri  HR, Gugliotta  JL, Borrelia miyamotoi disease: neither Lyme disease nor relapsing fever. Clin Lab Med. 2015;35:867–82. DOIPubMedGoogle Scholar
  4. Gugliotta  JL, Goethert  HK, Berardi  VP, Telford  SR III. Meningoencephalitis from Borrelia miyamotoi in an immunocompromised patient. N Engl J Med. 2013;368:240–5. DOIPubMedGoogle Scholar
  5. Hovius  JW, de Wever  B, Sohne  M, Brouwer  MC, Coumou  J, Wagemakers  A, A case of meningoencephalitis by the relapsing fever spirochaete Borrelia miyamotoi in Europe. Lancet. 2013;382:658. DOIPubMedGoogle Scholar
  6. Djukic  M, Schmidt-Samoa  C, Lange  P, Spreer  A, Neubieser  K, Eiffert  H, Cerebrospinal fluid findings in adults with acute Lyme neuroborreliosis. J Neurol. 2012;259:630–6. DOIPubMedGoogle Scholar
  7. Hytönen  J, Kortela  E, Waris  M, Puustinen  J, Salo  J, Oksi  J. CXCL13 and neopterin concentrations in cerebrospinal fluid of patients with Lyme neuroborreliosis and other diseases that cause neuroinflammation. J Neuroinflammation. 2014;11:103. DOIPubMedGoogle Scholar
  8. Tsutsumi  Y, Yamamoto  Y, Ito  S, Ohigashi  H, Shiratori  S, Naruse  H, Hepatitis B virus reactivation with a rituximab-containing regimen. World J Hepatol. 2015;7:2344–51. DOIPubMedGoogle Scholar
  9. Nei  M, Kumar  S. Molecular evolution and phylogenetics. New York: Oxford University Press; 2000.
  10. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9. DOIPubMedGoogle Scholar

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