Kateri Bertran, Charles Balzli, Yong-Kuk Kwon1
, Terrence M. Tumpey, Andrew Clark, and David E. Swayne
Figure 3. Histologic lesions and immunohistochemical detection of viral antigen in samples from ferrets exposed to live poultry market processing of highly pathogenic avian influenza A/Vietnam/1203/04 (H5N1) virus–infected chickens in study of airborne transmission of highly pathogenic influenza virus during processing of infected poultry. A) Olfactory bulb, 7 dpe, showing diffuse and severe neuropil malacia with mild cavitation and focal hemorrhages. Scale bar = 50 μm. B) Olfactory bulb, 7 dpe, showing viral antigen detected in neuropil, astrocytes, and neurons. Scale bar = 50 μm. C) Liver, 8 dpe, showing confluent coagulative necrosis of hepatocytes and bile duct necrosis with mononuclear cellular infiltrate in the portal triad. Scale bar = 50 μm. D) Liver, 8 dpe, showing viral antigen detected in hepatocytes, bile duct epithelia, and cellular debris. Scale bar = 50 μm. E) Nasal cavity, 7 dpe, showing moderate necrotic rhinitis with coagulative necrosis of mucous glandular epithelial cells; insert shows no viral antigen detected in mucosal membrane. Scale bars = 25 μm. F) Lung, 7 dpe, showing mild histiocytic interstitial pneumonia; insert shows viral antigen detected in type II pneumocytes. Scale bars = 25 μm. dpe, days postexposure.