Figure 1. Western blot analysis of proteinase K–resistant disease-associated prion protein (PrP^Sc) in tissue samples obtained from a cow at 88 months after oral inoculation with brain homogenate of L-type bovine spongiform encephalopathy (BSE) agent. The tissues tested are shown by lane: 1, olfactory bulb; 2, frontal cortex; 3, piriform cortex; 4, parietal cortex; 5, occipital cortex; 6, hippocampus; 7, putamen; 8, thalamus; 9, hypothalamus; 10, midbrain (superior colliculus); 11, obex; 12, cervical enlargement (C7) of spinal cord; 13, lumbar enlargement (L5) of spinal cord; 14, cerebellar cortex; 15, cerebellar white matter; 16, retina; 17, neurohypophysis; 18, trigeminal ganglion; 19, dorsal root ganglion (L5); 20, cervical cranial ganglion; 21, stellate ganglion; 22, celiac and mesenteric ganglion complex; 23, optic nerve; 24, cauda equina; 25, facial nerve; 26, hypoglossal nerve; 27, cervical vagus nerve; 28, sympathetic chain; 29, brachial nerve plexus; 30, sciatic nerve; 31, adrenal gland (medulla); 32, ileum; 33, colon. Lanes 1–16 and lanes 17–33 were loaded with 0.5 mg and 100 mg tissue equivalent, respectively. As controls, lanes L and C were also loaded with 0.5 mg of L-BSE and 0.17 mg of C-BSE cattle brain equivalent, respectively. The relative percentages of PrP^Sc (below each lane, upper panel) are normalized against midbrain. The PrP^Sc signals in the extracerebral tissues (below each lane, lower panel) are indicated as positive (+) or negative (–).